Description

2-[2-[2-[2-[6-(Biotinylaminohexanoyl]Aminoethoxy]Ethoxy]Ethoxy]-4-[3-(Trifluoromethyl)-3H-Diazirin-3-Yl]Benzoic Acid Hydrazide is a sophisticated bifunctional chemical probe designed for advanced photoaffinity labeling and bioconjugation studies. This compound integrates a biotin tag for high-affinity streptavidin capture with a photoreactive diazirine group for covalent crosslinking upon UV irradiation. It is essential for researchers and developers in the life sciences who require precise, irreversible tagging of biomolecules for target identification, protein-protein interaction mapping, and proteomic analysis.

Application

• Photoaffinity Labeling (PAL): Covalent capture of transient or weak protein-ligand interactions for target deconvolution and mechanism of action studies.
• Chemical Proteomics: Identification and profiling of protein targets and off-targets for drug candidates using mass spectrometry.
• Bioconjugation & Immobilization: Site-specific labeling of proteins, antibodies, or other biomolecules onto solid supports like beads or biosensor chips.
• Pull-down Assays: Isolation and purification of protein complexes from cell lysates via the biotin-streptavidin interaction.
• Activity-Based Protein Profiling (ABPP): Monitoring enzyme activity and occupancy within complex biological samples.
• Development of Diagnostic Reagents: Creating sensitive detection probes for immunoassays and biosensors.
• Structural Biology: Aiding in the study of macromolecular structures by providing crosslinking points for analysis.

Basic Information

Item	Detail
Product Name	2-[2-[2-[2-[6-(Biotinylaminohexanoyl]Aminoethoxy]Ethoxy]Ethoxy]-4-[3-(Trifluoromethyl)-3H-Diazirin-3-Yl]Benzoic Acid Hydrazide
CAS No.	1391054-06-0
Molecular Formula	C33H46F3N7O7S
Molecular Weight	741.82 g/mol
Synonyms	Biotin-PEG4-Benzophenone Hydrazide (Analog); Biotinylated Diazirine Crosslinker; Photoactivatable Biotin Probe; Biotin-PEG4-TFP-Azide (Related compound); Biotin Photoprobe; 4-(3-(Trifluoromethyl)diazirin-3-yl)-benzoic acid-PEG4-Biotin Hydrazide; Biotin-PEG4-Photoleucine; Biotinylated Aryl Diazirine
EINECS	Contact for details

Quality Control

Our 2-[2-[2-[2-[6-(Biotinylaminohexanoyl]Aminoethoxy]Ethoxy]Ethoxy]-4-[3-(Trifluoromethyl)-3H-Diazirin-3-Yl]Benzoic Acid Hydrazide is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and NMR/HRMS for structural confirmation, to ensure consistency and performance for sensitive research applications. Certificates of Analysis (COA) with detailed chromatograms and spectral data are provided to guarantee traceability and compliance with your research protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is both light-sensitive (store away from light) and hygroscopic (moisture-sensitive). For long-term stability, store under inert atmosphere (argon or nitrogen) and allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.