Description

Ws Dbco-Biotin CAS NO 1363444-70-5 is a high-purity, bifunctional chemical reagent designed for advanced bioconjugation and labeling applications. This compound is critical for researchers and manufacturers requiring precise, stable, and efficient linkage of biomolecules, such as antibodies or oligonucleotides, to detection or delivery systems. It is primarily utilized in the development of diagnostic assays, targeted drug delivery platforms, and advanced materials within the pharmaceutical, biotechnology, and life science research sectors.

Application

• Click Chemistry Conjugation: Serves as a key reagent for strain-promoted azide-alkyne cycloaddition (SPAAC) reactions, enabling the rapid and bioorthogonal labeling of azide-modified biomolecules.
• Diagnostic Probe Development: Used to create biotinylated detection probes for ELISA, lateral flow assays, and other immunoassay formats, leveraging the strong streptavidin-biotin interaction.
• ADC (Antibody-Drug Conjugate) Linker: Functions as a heterobifunctional linker in the synthesis of novel ADCs, connecting cytotoxic payloads to targeting antibodies.
• Biomaterial Functionalization: Employed to attach bioactive molecules (e.g., peptides, sugars) to surfaces, nanoparticles, or hydrogels for tissue engineering and biosensor applications.
• Fluorescent & Imaging Agent Synthesis: Conjugated to fluorophores or other imaging agents to create targeted probes for in vitro and in vivo imaging studies.
• Oligonucleotide Labeling: Facilitates the site-specific biotinylation of DNA or RNA strands for pull-down assays, sequencing, and microarray technologies.
• Affinity Chromatography Ligand: Used to immobilize biotinylated ligands onto streptavidin-coated resins for the purification of specific proteins or other molecules.

Basic Information

Product Name	Ws Dbco-Biotin
CAS No.	1363444-70-5
Molecular Formula	C33H39N5O5S
Molecular Weight	617.76 g/mol
Synonyms	DBCO-Biotin; DBCO-PEG4-Biotin; Dibenzocyclooctyne-PEG4-Biotin; (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl N-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanedioate; DBCO-SS-PEG4-Biotin (common trade name); Biotin-PEG4-DBCO; DBCO-PEG4-Biotin Conjugate; Azadibenzocyclooctyne-PEG4-Biotin
EINECS	Contact for details

Quality Control

Our Ws Dbco-Biotin is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment and NMR/MS for structural confirmation, to ensure it meets the high standards required for research and bioconjugation applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and other critical parameters is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.