Description

Neu5Acα(2-3)Galβ(1-3)Glcnac-β-Pnp is a synthetic, para-nitrophenyl (PNP)-tagged trisaccharide derivative, a crucial tool compound in glycobiology research. This molecule serves as a chromogenic substrate for the specific detection and kinetic analysis of glycosidase and glycosyltransferase enzymes. It is primarily utilized by researchers and process developers in the pharmaceutical, diagnostic, and biotechnology sectors for enzyme characterization, inhibitor screening, and assay development.

Application

• Enzyme Activity Assays: As a chromogenic substrate for α2-3 sialidases (neuraminidases) and other exo-glycosidases.
• High-Throughput Screening (HTS): For identifying and characterizing novel glycosidase inhibitors in drug discovery programs.
• Diagnostic Kit Development: Used in the formulation of enzymatic assays for clinical diagnostics and pathogen detection.
• Biochemical Research: Studying enzyme kinetics, specificity, and mechanism of action in carbohydrate-active enzymes.
• Quality Control: Monitoring enzyme purity and activity in biomanufacturing processes.
• Glycobiology Tool: Investigating sialylated glycan structures and their biological recognition events.

Basic Information

Product Name	Neu5Acα(2-3)Galβ(1-3)Glcnac-β-Pnp
CAS No.	1363424-95-6
Molecular Formula	C31H44N2O22
Molecular Weight	796.69 g/mol
Synonyms	5-N-Acetylneuraminyl-α-(2→3)-galactosyl-β-(1→3)-N-acetylglucosaminyl β-p-nitrophenyl; Neu5Ac-α-2,3-Gal-β-1,3-GlcNAc-β-PNP; PNP-β-Neu5Acα2-3Galβ1-3GlcNAc; Sialyl Lacto-N-biose I p-nitrophenyl glycoside; Chromogenic trisaccharide substrate; Sialidase substrate; Glycosidase substrate 1363424-95-6.
EINECS	Contact for details

Quality Control

Every batch of Neu5Acα(2-3)Galβ(1-3)Glcnac-β-Pnp is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure high purity and batch-to-batch consistency. Our quality assurance protocols are designed to meet the exacting standards of research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Specific Rotation	Contact for details
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.