Description

2-[N2-(4-Azido-2,3,5,6-Tetrafluorobenzoyl)-N6-(6-Biotinamidocaproyl)-L-Lysinyl]Ethyl 2-Carboxyethyl Disulfide is a sophisticated heterobifunctional crosslinking reagent designed for advanced bioconjugation and labeling applications. This compound matters because it integrates multiple functional handles—a photoactivatable aryl azide, a biotin affinity tag, and a cleavable disulfide bond—into a single molecule, enabling precise, multi-step biomolecular assembly and purification. It is primarily needed by researchers and developers in pharmaceutical R&D, proteomics, and diagnostic assay development for creating targeted probes and affinity-based capture systems.

Application

• Photoaffinity Labeling Probes: The aryl azide group allows for UV light-induced, non-selective crosslinking to biomolecules, ideal for mapping protein-protein or protein-ligand interaction sites.
• Biotin-Avidin Affinity Systems: The biotin moiety facilitates high-affinity capture and purification using streptavidin/avidin resins, a cornerstone technique in pull-down assays and diagnostics.
• Controlled Release Systems: The disulfide linker is cleavable under reducing conditions, enabling the elution of captured targets for downstream analysis.
• Surface Functionalization: Used to modify chips, beads, or other solid supports with biotin for creating sensor surfaces in SPR or microarray technologies.
• Antibody-Drug Conjugate (ADC) Research: Serves as a model linker-payload construct in early-stage research for developing cleavable ADC platforms.
• Chemical Biology Tool Development: A key reagent for synthesizing activity-based probes (ABPs) to study enzyme function and localization.

Basic Information

Product Name	2-[N2-(4-Azido-2,3,5,6-Tetrafluorobenzoyl)-N6-(6-Biotinamidocaproyl)-L-Lysinyl]Ethyl 2-Carboxyethyl Disulfide
CAS No.	1356841-73-0
Molecular Formula	C41H58F4N8O9S3
Molecular Weight	979.15 g/mol
Synonyms	Biotin-PEG3-Azide (Tetrafluorophenyl); Heterobifunctional Crosslinker, Biotin-Azide Disulfide; 4-Azido-2,3,5,6-tetrafluorobenzoic acid, compound with N6-(6-biotinamidocaproyl)-L-lysine ethyl 2-carboxyethyl disulfide; Biotinylated Photoactivatable Crosslinker; Biotin-(PEG)3-Azide (TFPA); Azide-PEG3-Biotin Disulfide Linker; Photo-leucine-biotin analog crosslinker
EINECS	Contact for details

Quality Control

Our production of this high-value biochemical reagent adheres to stringent GMP-grade principles to ensure batch-to-batch consistency and reliability for critical research. Identity and purity are verified by a combination of advanced analytical techniques including HPLC, mass spectrometry (MS), and NMR. A comprehensive Certificate of Analysis (COA) detailing purity, identity confirmation, and impurity profiles is provided with every shipment to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, consider under an inert atmosphere to preserve the reactivity of the azide and disulfide functional groups. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular ion [M+H]+
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.