Description

2-{N2-[N6-(4-Azido-2,3,5,6-Tetrafluorobenzoyl)-6-Aminocaproyl]-N6-(6-Biotinamidocaproyl)-L-Lysinylamido}]Ethyl Methanethiosulfonate is a sophisticated heterobifunctional crosslinking reagent designed for advanced bioconjugation and labeling applications. This compound integrates a photoactivatable aryl azide, a biotin affinity tag, and a thiol-reactive methanethiosulfonate group into a single molecule, enabling precise, multi-step attachment strategies. It is primarily utilized by researchers and developers in pharmaceutical R&D, proteomics, and diagnostic assay development for site-specific protein modification, affinity purification, and the construction of complex biomolecular assemblies.

Application

• Site-Specific Protein Labeling: The methanethiosulfonate group enables controlled, covalent attachment to cysteine residues for introducing biotin or other functionalities.
• Photoaffinity Labeling & Crosslinking: The tetrafluorophenyl azide moiety allows for UV light-induced, non-selective crosslinking to study protein-protein interactions or ligand binding sites.
• Affinity Purification & Pull-Down Assays: The biotin tag facilitates strong binding to streptavidin/avidin matrices for isolating and analyzing target biomolecules.
• Surface Immobilization: For functionalizing biosensor chips, microarrays, or nanoparticles with bioactive molecules in a controlled orientation.
• Proteomic Research: Used in mass spectrometry-based workflows for identifying and characterizing protein complexes and interaction networks.
• Antibody-Drug Conjugate (ADC) Research: Serves as a model or tool compound in developing novel linker-payload technologies for targeted therapies.
• Diagnostic Reagent Development: For creating highly specific detection probes and conjugates in immunoassays and molecular diagnostics.

Basic Information

Product Name	2-{N2-[N6-(4-Azido-2,3,5,6-Tetrafluorobenzoyl)-6-Aminocaproyl]-N6-(6-Biotinamidocaproyl)-L-Lysinylamido}]Ethyl Methanethiosulfonate
CAS No.	1356841-34-3
Molecular Formula	C41H58F4N10O8S2
Molecular Weight	951.09 g/mol
Synonyms	Biotin-PEG3-Azide-TFPA-MTS; Heterobifunctional Crosslinker (Biotin, Azide, MTS); 4-Azido-2,3,5,6-tetrafluorobenzoic acid, 6-[(6-{2-[(acetylthio)amino]ethyl}amino)-6-oxohexyl]amino]-6-oxohexyl ester, biotin conjugate; N2-[N6-(4-Azido-2,3,5,6-tetrafluorobenzoyl)-6-aminocaproyl]-N6-(6-biotinamidocaproyl)-L-lysinylamidoethyl methanethiosulfonate; Biotinylated Photoactivatable Crosslinker; MTS-Biotin-Azide Crosslinker; Biotin-PEG-Azide-TFPA Reagent
EINECS	Contact for details

Quality Control

This high-purity biochemical reagent is manufactured under strict quality control protocols. Each batch is characterized and tested to ensure identity, purity, and functional performance. A comprehensive Certificate of Analysis (COA) is provided, detailing results from analytical techniques including HPLC, NMR, and mass spectrometry. Our quality system is designed to support research and development requiring reproducible and reliable performance in sensitive applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.