Description

Biocytin-n-(T-Boc)-L-Proline is a specialized, high-purity biochemical building block designed for advanced research and synthesis. This compound matters for its role as a key intermediate in the development of biotinylated probes and peptide conjugates, enabling critical studies in molecular biology and diagnostics. It is primarily needed by pharmaceutical R&D teams, diagnostic kit manufacturers, and academic research institutions focused on affinity labeling, protein detection, and targeted drug delivery systems.

Application

• Synthesis of Biotinylated Peptides: A crucial precursor for attaching biotin tags to proline-containing peptide sequences for use in affinity purification and pull-down assays.
• Probe Development for Diagnostics: Used in the manufacture of high-sensitivity detection probes for ELISA, Western blotting, and immunohistochemistry applications.
• Avidin/Streptavidin Binding Studies: Serves as a model compound or intermediate in studying and optimizing biotin-streptavidin interaction kinetics.
• Chemical Biology Research: Enables site-specific labeling of proteins and enzymes in structural and functional studies.
• Precursor for Targeted Therapeutics: Employed in the research phase for developing biotin-drug conjugates for targeted cancer therapies.
• Material Surface Functionalization: Used to create biotin-coated surfaces for biosensors and microarray platforms.

Basic Information

Product Name	Biocytin-n-(T-Boc)-L-Proline
CAS No.	1356383-21-5
Molecular Formula	C25H42N4O7S
Molecular Weight	542.69 g/mol
Synonyms	N-α-(Biotinyl)-N-ε-(tert-Butoxycarbonyl)-L-lysyl-L-proline; Biotin-Lys(Boc)-Pro-OH; Biocytin, Nε-Boc-L-lysyl-L-proline derivative; (2S)-1-[(2S)-6-[(5-[(3aS,4S,6aR)-2-Oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoyl]amino]-2-[(2-methylpropan-2-yl)oxycarbonylamino]hexanoyl]pyrrolidine-2-carboxylic acid; Boc-Lys(Biotin)-Pro-OH; Biotinylated Boc-Lys-Pro
EINECS	Contact for details

Quality Control

Our Biocytin-n-(T-Boc)-L-Proline is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for research and development. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to cGMP guidelines for the production of advanced pharmaceutical intermediates, ensuring traceability and documentation for regulatory submissions.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Assay (HPLC)	≥ 95.0%
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.