Description

2-[N2-(4-Azido-2,3,5,6-Tetrafluorobenzoyl)-N6-(6-Biotinamidocaproyl)-L-Lysinyl]Ethyl Methanethiosulfonate is a sophisticated, multi-functional chemical reagent designed for advanced bioconjugation and labeling applications. Its molecular architecture combines a photoactivatable azide, a biotin affinity tag, and a methanethiosulfonate group for site-specific modification of cysteine residues, enabling precise cross-linking and detection strategies. This compound is essential for researchers and developers in proteomics, chemical biology, and diagnostic assay development, where high-purity, reliable conjugation tools are critical.

Application

• Site-Specific Protein Labeling: Covalent attachment of biotin or other probes to cysteine residues in proteins for structural and functional studies.
• Affinity Purification & Pull-Down Assays: Immobilization of target proteins or complexes via the biotin-streptavidin interaction for isolation and analysis.
• Cross-Linking & Protein-Protein Interaction Mapping: Photo-induced cross-linking (via the tetrafluorophenyl azide group) to capture transient or weak interactions in native biological systems.
• Diagnostic Probe Development: Construction of detection reagents for ELISA, Western blotting, and other immunoassays requiring stable, high-affinity tags.
• Surface Functionalization: Modification of biosensor chips, nanoparticles, or other solid supports with bioactive ligands for capture studies.
• Activity-Based Protein Profiling (ABPP): Design of probes to monitor enzyme activity or to identify active sites within complex proteomes.

Basic Information

Item	Details
Product Name	2-[N2-(4-Azido-2,3,5,6-Tetrafluorobenzoyl)-N6-(6-Biotinamidocaproyl)-L-Lysinyl]Ethyl Methanethiosulfonate
CAS No.	1356383-18-0
Molecular Formula	C36H50F4N8O7S2
Molecular Weight	846.96 g/mol
Synonyms	Biotin-PEG3-Azide-MTS; Biotinylated Photoactivatable Crosslinker; MTS-Biotin-Azide; 4-Azido-2,3,5,6-tetrafluorobenzoic acid, N-[6-[[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-1-oxopentyl]amino]-1-[2-[(methylsulfonyl)thio]ethoxy]-1-oxohexan-2-yl]amide; Biotin-LC-Azide-MTS; Photo-Leucine-Biotin Analog; Heterobifunctional Biotin-Azide Crosslinker
EINECS	Contact for details

Quality Control

Every batch of 2-[N2-(4-Azido-2,3,5,6-Tetrafluorobenzoyl)-N6-(6-Biotinamidocaproyl)-L-Lysinyl]Ethyl Methanethiosulfonate is manufactured and analyzed under strict quality systems. We ensure compliance with high-purity standards suitable for research and diagnostic applications. Comprehensive analytical data, including HPLC purity and identity confirmation (NMR, MS), is documented. A Certificate of Analysis (COA) is provided with each shipment to guarantee product integrity and performance.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.