Description

Dnp-Peg12-Nhs CAS NO 1334178-01-6 is a heterobifunctional crosslinker featuring a dinitrophenyl (DNP) group and an N-hydroxysuccinimide (NHS) ester, connected by a long, flexible polyethylene glycol (PEG) spacer. This compound is essential for creating stable, site-specific conjugations in bioconjugation and protein modification workflows, offering superior solubility and reduced steric hindrance compared to shorter linkers. It is primarily utilized by researchers and manufacturers in the fields of biopharmaceutical development, diagnostic assay production, and advanced materials science.

Application

• Antibody-Drug Conjugate (ADC) Development: For the controlled attachment of cytotoxic payloads to monoclonal antibodies via lysine residues.
• Diagnostic Reagent Conjugation: Linking enzymes (e.g., HRP, alkaline phosphatase) or fluorescent dyes to proteins and peptides for ELISA and immunoassay kits.
• Surface Functionalization: Modifying nanoparticles, biosensors, or microarray slides with specific ligands or capture molecules.
• Protein-Polymer Conjugation: Creating PEGylated proteins to enhance stability, solubility, and circulation half-life in therapeutic formulations.
• Affinity Labeling: Tagging proteins for purification, pull-down assays, or structural studies.
• Crosslinking for Structural Analysis: Studying protein-protein interactions and complex architectures.

Basic Information

Item	Detail
Product Name	Dnp-Peg12-Nhs
CAS No.	1334178-01-6
Molecular Formula	C45H73N3O20
Molecular Weight	976.07 g/mol
Synonyms	2,5-Dioxopyrrolidin-1-yl 3-(2-(2-(2-(2-(2-(2-(2-(2-(2-(2-(2-(2,4-dinitrophenoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)propanoate; DNP-PEG12-NHS ester; N-(Dinitrophenyl)-triethyleneglycol-N-hydroxysuccinimide ester (PEG12); DNP-PEG12-NHS Crosslinker; Heterobifunctional PEG Crosslinker, DNP and NHS; 1-[(2,4-Dinitrophenyl)-ω-hydroxy-pentaoxatetracosanoyloxy]-2,5-pyrrolidinedione; DNP-PEG12-OSu
EINECS	Contact for details

Quality Control

Our Dnp-Peg12-Nhs is manufactured under strict quality protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and structural confirmation by NMR and mass spectrometry. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing all specifications. Our quality system is designed to support research and cGMP-grade manufacturing requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange viscous oil or solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.