Description

Spdp-Peg3-Acid CAS NO 1334177-97-7 is a heterobifunctional crosslinker featuring a cleavable disulfide bond (SPDP) and a hydrophilic polyethylene glycol (PEG) spacer, terminated with a carboxylic acid group. This molecular architecture is critical for creating stable, yet reversible, conjugations between biomolecules, such as antibodies and enzymes, while improving solubility and reducing immunogenicity. It is an essential reagent for researchers and manufacturers in bioconjugation, antibody-drug conjugate (ADC) development, and diagnostic assay kit production.

Application

• Antibody-Drug Conjugate (ADC) Linker: For the controlled attachment of cytotoxic payloads to monoclonal antibodies via a cleavable disulfide linkage.
• Protein/Peptide Labeling: Conjugation of fluorescent dyes, enzymes, or other reporter molecules to proteins for diagnostic and imaging applications.
• Immobilization Chemistry: Covalent attachment of biomolecules to carboxyl-functionalized surfaces, beads, or biosensors.
• PEGylation Reagent: Introduction of a short, hydrophilic PEG spacer to enhance the pharmacokinetic properties of therapeutic proteins.
• Biomolecule Crosslinking: For studying protein-protein interactions or creating stable multi-component complexes.
• Liposome/ Nanoparticle Functionalization: Enabling targeted delivery systems by attaching targeting ligands to nanoparticle surfaces.

Basic Information

Product Name	Spdp-Peg3-Acid
CAS No.	1334177-97-7
Molecular Formula	C₂₀H₃₄N₂O₁₀S₂
Molecular Weight	526.6 g/mol
Synonyms	SPDP-PEG3-COOH; 3-(2-Pyridyldithio)propionyl-PEG3-acid; N-Succinimidyl 3-((2-(2-(3-(2-pyridyldithio)propanamido)ethoxy)ethoxy)ethyl)carbamate; Succinimidyl-[(N-maleimidopropionamido)-triethyleneglycol] ester; SMPT-PEG3-acid; LC-SPDP-PEG3-COOH; Heterobifunctional PEG crosslinker, SPDP-PEG3-acid
EINECS	Contact for details

Quality Control

Our Spdp-Peg3-Acid is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (NMR, MS), to ensure it meets the high standards required for sensitive bioconjugation work. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting purity, assay, and critical impurity profiles.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This compound is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95%
Assay	≥ 90%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.