Description

Alpha, ω-Disuccinimidyl Hexaethylene Glycol is a heterobifunctional, amine-reactive crosslinking reagent featuring a hydrophilic hexaethylene glycol spacer. This compound is essential for creating stable, well-defined conjugates between biomolecules, such as proteins and peptides, while maintaining solubility and reducing steric hindrance. It is a critical tool for researchers and manufacturers in bioconjugation, antibody-drug conjugate (ADC) development, and diagnostic assay formulation.

Application

• Bioconjugation and Protein Crosslinking: Primary application for linking amines on different biomolecules (e.g., antibody-enzyme conjugates).
• Antibody-Drug Conjugate (ADC) Linker Chemistry: Serves as a cleavable or non-cleavable spacer between the antibody and the cytotoxic payload.
• Diagnostic Reagent Development: Used to conjugate detection molecules (enzymes, fluorophores) to antibodies or antigens in ELISA and lateral flow assays.
• Surface Immobilization: Functionalizing solid supports (e.g., sensor chips, beads) with proteins or other amine-containing ligands.
• Polymer Modification: Introducing reactive NHS ester groups onto amine-terminated polymers for further functionalization.
• Peptide Tagging and Labeling: Attaching probes, tags, or other molecules to synthetic peptides for tracking or purification.

Basic Information

Product Name	Alpha, ω-Disuccinimidyl Hexaethylene Glycol
CAS No.	1334170-02-3
Molecular Formula	C₂₄H₃₈N₂O₁₂
Molecular Weight	546.57 g/mol
Synonyms	Hexaethylene glycol bis(succinimidyl succinate); NHS-PEG6-NHS; Bis-NHS-PEG6; Disuccinimidyl Hexaethylene Glycol Ester; PEG6 bis(NHS ester); DSS crosslinker analog (PEGylated); 1,11-Bis(2,5-dioxopyrrolidin-1-yloxy)-3,6,9-trioxaundecane-1,11-dioate; HO-PEG6-NHS (after hydrolysis of one end)
EINECS	Contact for details

Quality Control

Our Alpha, ω-Disuccinimidyl Hexaethylene Glycol is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency suitable for sensitive bioconjugation applications. Each lot is characterized by NMR, HPLC, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional performance is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid or powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.