Description

Nhs-Peg4-(m-Peg4)3-Ester CAS NO 1333154-70-3 is a heterobifunctional, multi-arm polyethylene glycol (PEG) crosslinker featuring an amine-reactive N-hydroxysuccinimide (NHS) ester group and multiple methoxy-PEG4 arms. This compound is engineered to provide controlled, multi-point conjugation, enhancing the stability and solubility of biomolecular constructs. It is a critical reagent for researchers and manufacturers in bioconjugation, pharmaceutical development, and advanced material science, enabling the creation of sophisticated drug delivery systems, diagnostic probes, and hydrogel networks.

Application

• Bioconjugation and Protein Modification: For site-specific labeling of antibodies, enzymes, and other proteins with dyes, drugs, or other functional molecules via primary amines (lysine residues).
• ADC (Antibody-Drug Conjugate) Linker: Serves as a sophisticated, branched linker to attach cytotoxic payloads to monoclonal antibodies, improving drug loading and pharmacokinetic profiles.
• PEGylation Reagent: Used to PEGylate peptides, proteins, and nanoparticles, significantly enhancing their solubility, stability, and circulation half-life in vivo.
• Hydrogel and Biomaterial Fabrication: Acts as a multi-functional crosslinking agent for creating biocompatible hydrogels with tunable mechanical properties for tissue engineering and 3D cell culture.
• Diagnostic Assay Development: Employed in the synthesis of detection probes and solid-phase assay components for ELISA, lateral flow tests, and other immunoassays.
• Surface Functionalization: Used to modify surfaces (e.g., gold nanoparticles, chips, beads) with amine-reactive groups for subsequent biomolecule immobilization.
• Polymer Chemistry: A key building block for synthesizing complex, branched, and star-shaped polymers with precise architecture.

Basic Information

Product Name	Nhs-Peg4-(m-Peg4)3-Ester
CAS No.	1333154-70-3
Molecular Formula	C58H104N4O28
Molecular Weight	1305.5 g/mol (approx.)
Synonyms	NHS-PEG4-(mPEG4)3; (mPEG4)3-PEG4-NHS Ester; N-Hydroxysuccinimide ester of (mPEG4)3-PEG4-acid; Tetraethylene glycol) derivative, 2,5-dioxo-1-pyrrolidinyl ester; 4-Arm PEG-NHS, Branched PEG-NHS Ester; Heterobifunctional PEG Crosslinker, NHS-(PEG4)4; PEG-based NHS-activated crosslinker
EINECS	Contact for details

Quality Control

Every batch of Nhs-Peg4-(m-Peg4)3-Ester is manufactured under strict quality management systems and undergoes comprehensive analytical characterization. We ensure high lot-to-lot consistency and purity suitable for sensitive research and development applications. Certificates of Analysis (COA) detailing purity, identity, and functional group integrity are provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis of the active NHS ester group.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Group Substitution	≥ 90% (NHS ester)
Solubility	Soluble in DMSO, DMF, acetonitrile, moderately soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.