Description

Mal-PEG4-NHS Ester is a heterobifunctional crosslinker featuring a maleimide group and an N-hydroxysuccinimide (NHS) ester, connected by a tetraethylene glycol (PEG4) spacer. This compound is essential for creating stable, site-specific conjugates between thiol-containing molecules (like cysteine residues in proteins or peptides) and primary amines, offering enhanced solubility and reduced aggregation. It is a critical reagent for researchers and manufacturers in bioconjugation, antibody-drug conjugate (ADC) development, and peptide synthesis.

Application

• Antibody-Drug Conjugate (ADC) Linker: Site-specific conjugation of cytotoxic payloads to monoclonal antibodies via cysteine residues.
• Protein and Peptide Labeling: Attachment of fluorescent dyes, biotin, or other probes to thiol-containing biomolecules.
• PEGylation Reagent: Introduction of a hydrophilic PEG spacer to improve the pharmacokinetic properties of therapeutic proteins and peptides.
• Surface Functionalization: Modification of gold nanoparticles or other surfaces with thiol chemistry for biosensor and diagnostic assay development.
• Enzyme Immobilization: Covalent attachment of enzymes to solid supports for biocatalysis and industrial processes.
• Small Molecule Conjugation: Creating targeted drug delivery systems by linking drug molecules to targeting ligands.

Basic Information

Product Name	Mal-PEG4-NHS Ester
CAS No.	1325208-25-0
Molecular Formula	C21H30N2O10
Molecular Weight	470.47 g/mol
Synonyms	Maleimide-PEG4-NHS ester; Maleimido-PEG4-NHS; NHS-PEG4-Maleimide; 3-Maleimidopropionic acid-PEG4-NHS ester; 3-Maleimidopropionamido-PEG4-NHS ester; N-(3-Maleimidopropionyl)-3,6,9,12-tetraoxapentadecan-15-oic acid succinimidyl ester; Mal-PEG4-OSu; PEG4-(NHS ester)-(C3-Maleimide)
EINECS	Contact for details

Quality Control

Our Mal-PEG4-NHS Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC purity analysis, NMR confirmation, and functional group validation, to ensure consistency and performance. Certificates of Analysis (COA) with detailed specifications are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.