Description

5'-Cholesteryl-Teg Phosphoramidite is a specialized, high-purity building block used in the automated synthesis of oligonucleotides. This compound is critical for introducing a cholesterol moiety via a tetraethylene glycol (TEG) linker, enabling the creation of modified nucleic acids with enhanced cellular uptake and stability. It is an essential reagent for researchers and manufacturers in the fields of therapeutic oligonucleotide development, diagnostic probe synthesis, and advanced genetic research.

Application

• Therapeutic Oligonucleotide Synthesis: Key building block for producing cholesterol-conjugated antisense oligonucleotides (ASOs), siRNA, and aptamers to improve pharmacokinetics and target delivery.
• Diagnostic Probe Development: Used to manufacture labeled probes for fluorescence in situ hybridization (FISH) and other detection assays, where the cholesterol tag aids in membrane association.
• Lipid Nanoparticle (LNP) Formulation Research: Serves as a functional component in the study and development of novel LNP systems for nucleic acid delivery.
• Basic Biochemical Research: Enables the study of membrane-protein interactions, cellular trafficking, and the structure-activity relationship of modified nucleic acids.
• Synthesis of Cholesterol-Tagged Primers and Linkers: For specialized PCR applications and the construction of complex molecular architectures.

Basic Information

Product Name	5'-Cholesteryl-Teg Phosphoramidite
CAS No.	1325147-19-0
Molecular Formula	C56H95N2O7P
Molecular Weight	939.34 g/mol
Synonyms	Cholesterol-TEG Phosphoramidite; 5'-O-(4,4'-Dimethoxytrityl)-N6-cholesteryl-2'-deoxyadenosine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 5'-Cholesteryl-TEG-CE Phosphoramidite; Cholesterol Tetraethylene Glycol Phosphoramidite; 1-O-[2-Cyanoethoxy(diisopropylamino)phosphino]-2-O-(cholesteryl-3-succinyl)-3-O-(4,4'-dimethoxytrityl)-triethyleneglycol; DMT-Cholesterol-TEG Phosphoramidite
EINECS	Contact for details

Quality Control

Every batch of our 5'-Cholesteryl-Teg Phosphoramidite is manufactured and tested under strict quality management systems. We guarantee high purity and lot-to-lot consistency through comprehensive analytical techniques including HPLC, NMR, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming identity, purity, and suitability for oligonucleotide synthesis.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and should be handled under dry conditions to maintain stability and reactivity.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in anhydrous acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.