Description

Fmoc-Peg4-Nhs Ester is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an amine-reactive N-hydroxysuccinimide (NHS) ester and an acid-labile Fmoc-protected amine. This compound is essential for creating stable, hydrophilic, and cleavable bioconjugates, particularly in the development of advanced drug delivery systems and diagnostic reagents. It is a critical building block for researchers and manufacturers in the pharmaceutical, biotech, and life sciences sectors who require precise and reversible linkage strategies.

Application

• Bioconjugation and Linker Chemistry: Used to attach peptides, proteins, or other biomolecules to surfaces, nanoparticles, or other molecules via its NHS ester group, with the Fmoc group providing a protected handle for further orthogonal chemistry.
• ADC (Antibody-Drug Conjugate) Development: Serves as a cleavable, hydrophilic spacer to connect cytotoxic payloads to monoclonal antibodies, improving solubility and pharmacokinetics while allowing for controlled drug release.
• PEGylation Reagent: Functions as a versatile PEGylation agent to modify therapeutic proteins and peptides, enhancing their stability, solubility, and circulation half-life in vivo.
• Solid-Phase Peptide Synthesis (SPPS): Employed as a building block to introduce a hydrophilic, cleavable PEG spacer during the synthesis of complex peptides and peptidomimetics.
• Surface Functionalization: Utilized to create functionalized surfaces for biosensors, microarrays, and diagnostic assays by providing a reactive NHS ester for covalent immobilization of biomolecules.
• Prodrug Synthesis: Applied in the design of prodrugs where a therapeutic agent is linked via the cleavable Fmoc-Peg4 spacer for targeted activation.
• Materials Science: Incorporated into hydrogels and polymeric materials to introduce biodegradable linkages and reactive sites for further modification.

Basic Information

Product Name	Fmoc-Peg4-Nhs Ester
CAS No.	1314378-14-7
Molecular Formula	C₃₀H₄₄N₂O₁₁
Molecular Weight	608.68 g/mol
Synonyms	Fmoc-N-amido-PEG4-NHS ester; N-(((9H-Fluoren-9-yl)methoxy)carbonyl)-1-amino-3,6,9,12-tetraoxapentadecan-15-oic acid 2,5-dioxopyrrolidin-1-yl ester; Fmoc-15-amino-4,7,10,13-tetraoxapentadecanoic acid NHS ester; Fmoc-PEG4-OSu; Fmoc-Amino-PEG4-NHS Ester; Fmoc-NH-PEG4-NHS; 1-[(9H-Fluoren-9-ylmethoxy)carbonylamino]-3,6,9,12-tetraoxapentadecan-15-oic acid N-succinimidyl ester
EINECS	Contact for details

Quality Control

Our Fmoc-Peg4-Nhs Ester is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity for research and cGMP applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via NMR and mass spectrometry. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and the NHS ester group is susceptible to hydrolysis. For long-term storage, keep under an inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95% (Area %)
Water Content (KF)	≤ 1.0 %
Solubility	Soluble in DMF, DMSO, acetonitrile; partially soluble in dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.