Description

Azido-PEG2-NHS Ester is a heterobifunctional crosslinking reagent featuring an amine-reactive N-hydroxysuccinimide (NHS) ester and a click chemistry-ready azide group. This compound is essential for creating stable, site-specific conjugates between biomolecules and surfaces or other molecules under mild, aqueous conditions. It is primarily used by researchers and manufacturers in bioconjugation, pharmaceutical development, and materials science for applications such as antibody-drug conjugate (ADC) synthesis and surface functionalization.

Application

• Bioconjugation and ADC Development: Site-specific conjugation of drugs, fluorophores, or other payloads to antibodies, proteins, or peptides via the azide-alkyne cycloaddition (click chemistry).
• Surface Functionalization: Immobilization of biomolecules onto azide-modified surfaces, nanoparticles, or polymers for biosensor and diagnostic assay development.
• Polymer and Biomaterial Modification: Introduction of azide functionalities into polymers or hydrogels for subsequent bio-orthogonal labeling or crosslinking.
• Chemical Biology Probes: Synthesis of activity-based probes or affinity tags for target identification and proteomics studies.
• Nucleic Acid Labeling: Conjugation of reporter molecules to amine-modified oligonucleotides for sequencing or detection applications.
• Glycan and Carbohydrate Chemistry: Labeling of glycoproteins or synthetic carbohydrates for structural and functional analysis.

Basic Information

Item	Detail
Product Name	Azido-PEG2-NHS Ester
CAS No.	1312309-64-0
Molecular Formula	C₁₃H₁₉N₅O₆
Molecular Weight	341.32 g/mol
Synonyms	Azido-PEG2-NHS ester; 2-[2-[2-(2-Azidoethoxy)ethoxy]ethoxy]ethyl N-succinimidyl carbonate; Azido-PEG2-O-COO-NHS; NHS-PEG2-Azide; Azido-PEG2-succinimidyl carbonate; 2,5-Dioxopyrrolidin-1-yl 2-[2-[2-(2-azidoethoxy)ethoxy]ethoxy]ethyl carbonate; Azido-PEG2-OSu; PEG2-Azide-NHS Ester
EINECS	Contact for details

Quality Control

Our Azido-PEG2-NHS Ester is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research and development applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional group integrity. We adhere to ISO 9001 quality management principles, and our production environment is designed to meet the stringent requirements of GMP for pharmaceutical intermediates.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or oil
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.