Description

Desthiobiotin-Peg3-Azide is a high-purity, functionalized biotin derivative designed for advanced bioconjugation and chemical biology applications. This compound is engineered to facilitate efficient and specific labeling of biomolecules through copper-free click chemistry, offering superior stability and versatility compared to standard biotin-azide reagents. It is an essential tool for researchers and developers in pharmaceutical R&D, proteomics, and diagnostic assay development, enabling the creation of targeted probes and affinity matrices.

Application

• Bioconjugation and Probe Development: Used as a key reagent for site-specific labeling of proteins, antibodies, and oligonucleotides via strain-promoted azide-alkyne cycloaddition (SPAAC).
• Affinity Purification Systems: Serves as a linker for immobilizing ligands onto solid supports (e.g., agarose beads) to create streptavidin/avidin-based purification columns.
• Chemical Biology and Target Identification: Enables the synthesis of activity-based probes (ABPs) for profiling enzyme activity and identifying drug targets in complex biological systems.
• Diagnostic Assay Manufacturing: Incorporated into the development of ELISA, lateral flow assays, and other immunoassays for highly sensitive detection.
• Cell Surface Engineering and Imaging: Facilitates the labeling of cell surface glycans and proteins for fluorescence-activated cell sorting (FACS) and microscopy studies.
• ADC (Antibody-Drug Conjugate) Linker Exploration: Acts as a model or functional component in the research and development of novel linker-payload systems for targeted cancer therapies.
• Materials Science and Nanotechnology: Used to functionalize nanoparticles, polymers, and surfaces with biotin for creating smart biomaterials and biosensors.

Basic Information

Item	Details
Product Name	Desthiobiotin-Peg3-Azide
CAS No.	1306615-47-3
Molecular Formula	C₂₂H₄₀N₆O₆S
Molecular Weight	516.66 g/mol
Synonyms	Desthiobiotin-PEG3-azide; 5-[(3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]-N-(2-{2-[2-(2-azidoethoxy)ethoxy]ethoxy}ethyl)pentanamide; Azido-PEG3-desthiobiotin; N-(Azido-PEG3)-desthiobiotin; Biotin-PEG3-azide (desthiobiotin variant); 1-(2-{2-[2-(2-Azidoethoxy)ethoxy]ethoxy}ethyl)-2-oxo-hexahydro-1H-thieno[3,4-d]imidazole-4-pentanamide
EINECS	Contact for details

Quality Control

Every batch of Desthiobiotin-Peg3-Azide is manufactured under strict quality management systems and undergoes comprehensive analytical testing. We ensure compliance with high-purity standards suitable for research and in-vitro diagnostic applications. A detailed Certificate of Analysis (COA) providing data on purity, identity, and impurities is supplied with each shipment to guarantee batch-to-batch consistency and traceability.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccated conditions. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.