Description

15-(2-Pyridyldithio)-4,7,10,13-Tetraoxapentadecanoic Acid, n-Hydroxysuccinimide Ester is a heterobifunctional crosslinking reagent featuring a reactive NHS ester and a cleavable pyridyldithiol group. This compound is critical for creating stable, yet selectively reversible, conjugates between amine-containing molecules and thiol groups, enabling advanced bioconjugation strategies. It is an essential tool for researchers and manufacturers in biopharmaceutical development, diagnostic assay production, and advanced materials science, where precise and controllable molecular linkage is required.

Application

• Antibody-Drug Conjugate (ADC) Linker: For the site-specific conjugation of cytotoxic payloads to monoclonal antibodies via lysine residues.
• Protein/Peptide Labeling: To introduce fluorescent dyes, biotin, or other probes onto proteins for imaging and detection applications.
• Surface Functionalization: For immobilizing biomolecules (e.g., proteins, peptides) onto biosensor chips, microarrays, or nanoparticles via amine-reactive chemistry.
• Polymer Modification: In the synthesis of smart polymers and hydrogels with cleavable disulfide linkages for controlled release systems.
• Liposome and Nanoparticle Conjugation: To attach targeting ligands (e.g., antibodies, peptides) to drug delivery carriers.
• Enzyme Immobilization: For creating stable, yet potentially reversible, enzyme conjugates used in biocatalysis and industrial processes.
• Crosslinking Studies: Used in structural biology to study protein-protein interactions and complex formations.
• Diagnostic Reagent Synthesis: A key intermediate in the production of reagents for immunoassays and lateral flow tests.

Basic Information

Item	Details
Product Name	15-(2-Pyridyldithio)-4,7,10,13-Tetraoxapentadecanoic Acid, n-Hydroxysuccinimide Ester
CAS No.	1305053-43-3
Molecular Formula	C22H28N2O9S2
Molecular Weight	528.60 g/mol
Synonyms	SPDP-PEG4-NHS ester; NHS-PEG4-SPDP; 2,5-Dioxopyrrolidin-1-yl 15-(2-pyridinyldithio)-4,7,10,13-tetraoxapentadecanoate; PEG4-SPDP crosslinker; Heterobifunctional PEG crosslinker, NHS-Pyridyldithiol; Amine and sulfhydryl reactive crosslinker; Succinimidyl ester of SPDP-PEG4-acid
EINECS	Contact for details

Quality Control

Our 15-(2-Pyridyldithio)-4,7,10,13-Tetraoxapentadecanoic Acid, n-Hydroxysuccinimide Ester is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive bioconjugation applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and the NHS ester group is susceptible to hydrolysis. For long-term storage, keep containers sealed under an inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Assay	≥ 90% (NHS ester active group)
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.