Description

5-Bromo-4-Chloro-3-Indolyl-β-D-Fucopyranoside is a specialized chromogenic substrate used for the detection of specific enzymatic activity. This compound is critical for research and diagnostic applications where precise biochemical visualization is required. It is primarily utilized by scientists and manufacturers in the fields of molecular biology, diagnostic assay development, and carbohydrate research.

Application

• Enzyme Substrate in Histochemistry & Cytochemistry: Used as a chromogenic substrate for β-D-fucosidase in staining procedures to visualize enzyme localization in tissue sections and cell cultures.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits and reagents for detecting specific bacterial or human enzyme markers.
• Biochemical Research: Employed in enzymatic assays to study the kinetics, inhibition, and specificity of glycoside hydrolases, particularly β-fucosidases.
• Microbiology Applications: Used in differential media and identification systems for microbiological analysis, helping to distinguish between microbial species based on their enzymatic profiles.
• Glycobiology Studies: Serves as a tool for investigating glycosidase pathways and carbohydrate metabolism in various biological systems.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indolyl-β-D-Fucopyranoside
CAS No.	1301706-80-8
Molecular Formula	C14H15BrClNO6
Molecular Weight	408.63 g/mol
Synonyms	X-β-D-Fuc; 5-Bromo-4-chloro-3-indolyl β-D-fucopyranoside; BCIP-β-D-Fucose; 5-Br-4-Cl-3-indolyl β-D-fucoside; β-D-Fucosidase substrate; Chromogenic β-D-fucosidase substrate; Indoxyl-β-D-fucopyranoside derivative
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indolyl-β-D-Fucopyranoside is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard assays. We adhere to ISO 9001 quality management standards, and our production environment is designed to prevent cross-contamination.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. This product is hygroscopic (moisture-sensitive) and should be handled in a low-humidity environment when opened to prevent degradation. For long-term storage, consider desiccant use.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Heavy Metals	≤ 20 ppm
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Performance in Enzyme Assay	Conforms to reference activity

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.