Description

8-(8-Biotinylamino-3,6-Dioxa-Octanoylamino)-3,6-Dioxa-Octanoic Acid is a sophisticated, heterobifunctional cross-linking reagent designed for advanced bioconjugation. This compound matters because it provides a cleavable, hydrophilic PEG-based spacer arm, enabling the stable attachment of biotin to target molecules for sensitive detection and purification applications. It is essential for researchers and manufacturers in the fields of proteomics, diagnostics, and pharmaceutical development who require high-purity, reliable linkers for assay and sensor development.

Application

• Bioconjugation Reagent: For attaching biotin tags to antibodies, proteins, peptides, and other biomolecules.
• Diagnostic Assay Development: Used in the fabrication of ELISA, lateral flow assays, and other immunoassays requiring streptavidin-biotin chemistry.
• Affinity Chromatography: Immobilization ligand for creating streptavidin/avidin-based purification columns and resins.
• Cell Surface Labeling: Modification of cell surface proteins or carbohydrates for flow cytometry and microscopy studies.
• Drug Delivery Systems: As a component in the design of targeted therapeutic conjugates.
• Microarray and Biosensor Fabrication: For the controlled immobilization of capture molecules on solid surfaces.
• Pull-Down Assays: Essential tool in proteomics for isolating and identifying protein complexes.
• Nucleic Acid Probes: Biotinylation of oligonucleotides for hybridization-based detection methods.

Basic Information

Item	Details
Product Name	8-(8-Biotinylamino-3,6-Dioxa-Octanoylamino)-3,6-Dioxa-Octanoic Acid
CAS No.	1301706-65-9
Molecular Formula	C26H44N4O10S
Molecular Weight	604.71 g/mol
Synonyms	Biotin-PEG3-acid; Biotin-PEG3-COOH; Biotin-PEG3-Acid; Biotin-PEG3-Propionic Acid; Biotin-amide-PEG3-acid; 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(17-carboxy-3,6,9,12,15-pentaoxaheptadecan-1-yl)pentanamide; Biotinamido-PEG3-acid; EZ-Link Biotin-PEG3-Acid
EINECS	Contact for details

Quality Control

Our 8-(8-Biotinylamino-3,6-Dioxa-Octanoylamino)-3,6-Dioxa-Octanoic Acid is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for life science research. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Assay	≥ 90.0% (on anhydrous basis)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.