Description

n-(3-Methylcrotonyl)Glycine-2,2-D2 is a deuterium-labeled derivative of the endogenous metabolite n-(3-methylcrotonyl)glycine. This stable isotope-labeled compound is essential for precise analytical and metabolic studies, offering a non-radioactive tracer with minimal isotopic interference. It is primarily utilized by researchers and manufacturers in the fields of pharmaceutical development, clinical diagnostics, and advanced metabolomics for use as an internal standard in mass spectrometry.

Application

• Internal Standard for LC-MS/MS Analysis: Critical for the accurate quantification of endogenous n-(3-methylcrotonyl)glycine in biological samples for research and diagnostic assays.
• Metabolomics Research: Used as a stable isotope tracer to study metabolic pathways, particularly those related to leucine catabolism and inborn errors of metabolism.
• Biomarker Discovery and Validation: Enables high-precision measurement in studies aiming to identify and validate n-(3-methylcrotonyl)glycine as a clinical biomarker.
• Pharmaceutical R&D: Supports drug metabolism and pharmacokinetics (DMPK) studies for compounds affecting relevant metabolic pathways.
• Quality Control Reference Material: Serves as a certified reference standard for in-vitro diagnostic (IVD) kit manufacturers and clinical laboratories.
• Isotope Dilution Mass Spectrometry (IDMS): The gold-standard method for achieving ultimate accuracy in quantitative analysis, reliant on high-purity labeled standards like this.

Basic Information

Product Name	n-(3-Methylcrotonyl)Glycine-2,2-D2
CAS No.	1276197-31-9
Molecular Formula	C7H9D2NO3
Molecular Weight	159.18 g/mol (approx.)
Synonyms	3-Methylcrotonylglycine-2,2-d2; (3-Methylcrotonyl)glycine-2,2-d2; 2-[(3-Methylbut-2-enoyl)amino]acetic-2,2-d2 acid; N-(3-Methyl-2-butenoyl)glycine-2,2-d2; 3-Methylcrotonylglycine-d2 (2,2); Deuterated 3-Methylcrotonylglycine; 3-MCG-d2; Stable Isotope Labeled 3-Methylcrotonylglycine
EINECS	Contact for details

Quality Control

Every batch of n-(3-Methylcrotonyl)Glycine-2,2-D2 is manufactured under controlled conditions and undergoes rigorous analytical characterization to ensure identity, purity, and isotopic enrichment. Our quality assurance protocol includes comprehensive testing via NMR, HPLC, and Mass Spectrometry. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming specifications for chemical purity, isotopic purity (D-enrichment), and absence of critical impurities. We support compliance with ISO 17034 standards for reference material producers.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as specified on the label or COA. This compound is hygroscopic (moisture-sensitive); the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term storage, consider desiccants or inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-enrichment)	≥ 98.0 atom % D
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.