Description

(2,5-Dioxopyrrolidin-1-Yl) 3-[2-[2-[6-(2,5-Dioxopyrrol-1-Yl)Hexanoylamino]Ethoxy]Ethoxy]Propanoate is a heterobifunctional crosslinking agent featuring both NHS ester and maleimide reactive groups. This compound is engineered for the efficient and site-specific conjugation of biomolecules, enabling the creation of stable amide and thioether bonds. It is an essential tool for researchers and manufacturers in bioconjugation, antibody-drug conjugate (ADC) development, and advanced diagnostics, where precise and reliable protein modification is critical.

Application

• Antibody-Drug Conjugate (ADC) Linker: A key intermediate for connecting cytotoxic payloads to monoclonal antibodies via lysine residues.
• Protein-Protein/Peptide Conjugation: Facilitates the crosslinking of proteins or peptides for structural studies, immunoassays, and the development of bispecific constructs.
• Surface Immobilization: Used to functionalize sensor chips, nanoparticles, or solid supports with proteins or other amine-containing ligands for biosensing and diagnostic applications.
• Fluorescent Probe & Label Synthesis: Conjugation of fluorescent dyes or other reporter molecules to antibodies, enzymes, or oligonucleotides for imaging and detection.
• Polymer & Biomaterial Modification: Incorporation of bioactive molecules into hydrogels, polymers, or drug delivery systems to enhance targeting or functionality.
• Enzyme & Catalyst Immobilization: Attachment of enzymes to various matrices for use in biocatalysis and industrial enzymatic processes.

Basic Information

Product Name	(2,5-Dioxopyrrolidin-1-Yl) 3-[2-[2-[6-(2,5-Dioxopyrrol-1-Yl)Hexanoylamino]Ethoxy]Ethoxy]Propanoate
CAS No.	1263044-56-9
Molecular Formula	C23H32N4O10
Molecular Weight	524.52 g/mol
Synonyms	NHS-PEG2-Maleimide; Maleimide-PEG2-NHS ester; SM(PEG)2; NHS-PEG2-MAL; 6-Maleimidocaproic acid NHS-PEG2 ester; 3-[2-[2-[6-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)hexanamido]ethoxy]ethoxy]propanoic acid 2,5-dioxopyrrolidin-1-yl ester; 1-[6-({2-[2-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethoxy]ethoxy}acetyl)amino]hexyl]-1H-pyrrole-2,5-dione 2,5-dioxopyrrolidin-1-yl ester
EINECS	Contact for details

Quality Control

Our bioconjugation linkers are produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry for identity verification. We provide Certificates of Analysis (COA) with detailed specifications to ensure traceability and support your research and development needs. Our commitment to high-purity, low endotoxin, and reproducible performance is paramount for critical applications like ADC development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.