Description

25-Hydroxy Vd2-D6 is a high-purity, deuterium-labeled internal standard critical for accurate quantitative analysis in mass spectrometry. This compound is essential for researchers and quality control laboratories requiring precise measurement of vitamin D metabolites. It is primarily used in pharmaceutical development, clinical diagnostics, and advanced nutritional research where method validation and regulatory compliance are paramount.

Application

• LC-MS/MS Internal Standard: For the precise quantification of 25-hydroxyvitamin D2 and related metabolites in biological matrices.
• Clinical Diagnostics: Used in the development and validation of assays for vitamin D status testing in human serum and plasma.
• Pharmaceutical R&D: Supports pharmacokinetic studies and bioanalytical method development for vitamin D-based therapeutics.
• Nutritional Research: Enables accurate analysis of vitamin D metabolites in fortified foods, supplements, and metabolic studies.
• Reference Material: Serves as a certified reference standard for calibrating analytical instrumentation and ensuring data integrity.
• Forensic Toxicology: Applied in specialized toxicology panels for the accurate assessment of vitamin D levels.

Basic Information

Product Name	25-Hydroxy Vd2-D6
CAS No.	1262843-46-8
Molecular Formula	C28H40D6O3
Molecular Weight	~428.7 g/mol
Synonyms	25-Hydroxyergocalciferol-d6; (3β,5Z,7E,22E)-9,10-Secoergosta-5,7,10(19),22-tetraen-3-ol-d6; 25(OH)D2-d6; Vitamin D2 Metabolite-d6; 25-Hydroxyvitamin D2-d6; Ercalcidiol-d6; Internal Standard for 25-OH-D2
EINECS	Contact for details

Quality Control

Every batch of 25-Hydroxy Vd2-D6 is manufactured under strict quality management systems and undergoes comprehensive analytical characterization. Our products are validated for use as internal standards with guaranteed isotopic purity and chemical stability. Certificates of Analysis (COA) detailing results from NMR, HPLC, and MS are provided to ensure traceability and compliance with your research or quality protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. Due to its hygroscopic (moisture-sensitive) nature, the container should be allowed to reach room temperature before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (MS)	≥ 98.0 atom % D
Chemical Purity	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in ethanol, methanol, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.