Description

6β-Hydroxy Cortisol-D4 is a high-purity, deuterium-labeled internal standard critical for advanced analytical quantification. This stable isotope-labeled compound is essential for ensuring the accuracy and reliability of mass spectrometry-based assays by correcting for analyte loss during sample preparation and instrument variability. It is primarily utilized by pharmaceutical R&D laboratories, clinical research organizations, and bioanalytical service providers for the precise measurement of endogenous cortisol and its metabolites in complex biological matrices.

Application

• Quantitative Bioanalysis: Serves as an indispensable internal standard in LC-MS/MS and GC-MS methods for the accurate quantification of 6β-hydroxycortisol and related steroid hormones in plasma, serum, and urine.
• Drug Metabolism & Pharmacokinetics (DMPK): Used in studies to assess cytochrome P450 3A4 (CYP3A4) enzyme activity, a key indicator of drug-drug interactions and metabolic pathways.
• Clinical Diagnostics & Research: Enables precise measurement in research concerning adrenal function, Cushing's syndrome, and other endocrine disorders.
• Method Development & Validation: Critical for developing and validating robust, regulatory-compliant (FDA, EMA) bioanalytical methods in pharmaceutical development.
• Quality Control in Testing Labs: Provides a reference standard for ensuring the consistency and accuracy of routine testing in commercial and hospital laboratories.

Basic Information

Product Name	6β-Hydroxy Cortisol-D4
CAS No.	1261254-51-6
Molecular Formula	C₂₁H₂₆D₄O₆
Molecular Weight	370.48 g/mol
Synonyms	6β-Hydroxycortisol-d4; 6β,11β,17α,21-Tetrahydroxypregn-4-ene-3,20-dione-19,19,21,21-d4; 11β,17α,21-Trihydroxy-6β-hydroxypregn-4-ene-3,20-dione-19,19,21,21-d4; Deuterated 6β-Hydroxycortisol; Cortisol-6β-ol-d4; [19,19,21,21-d4]-6β-Hydroxycortisol
EINECS	Contact for details

Quality Control

Every batch of 6β-Hydroxy Cortisol-D4 is manufactured and tested under strict quality management systems. We provide comprehensive Certificates of Analysis (COA) detailing purity (typically ≥98% by HPLC), isotopic enrichment, and identity confirmation. Our quality protocols are designed to meet the stringent requirements of pharmaceutical R&D and regulatory bioanalysis, ensuring reliable performance as a critical reference material.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Chemical Purity (NMR)	Conforms
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.