Description

17β-Estradiol-[2,3,4-13C3] is a stable isotope-labeled analog of the primary female sex hormone, estradiol, specifically enriched with carbon-13 at the 2, 3, and 4 positions. This compound is an essential internal standard and reference material for high-precision quantitative analysis in mass spectrometry-based assays. It is primarily used by research institutions, pharmaceutical companies, and contract research organizations (CROs) engaged in bioanalytical method development, pharmacokinetic studies, and clinical diagnostics.

Application

• Quantitative Bioanalysis: Serves as a critical internal standard for the accurate LC-MS/MS quantification of endogenous estradiol levels in biological matrices such as plasma, serum, and tissue homogenates.
• Pharmacokinetic/Pharmacodynamic (PK/PD) Studies: Enables precise tracking of drug metabolism and hormone dynamics in preclinical and clinical research for hormone replacement therapies and endocrine-disrupting compounds.
• Clinical Diagnostics: Used in the development and validation of high-sensitivity diagnostic assays for monitoring hormonal disorders, fertility treatments, and certain cancers.
• Metabolic Pathway Tracing: The 13C-labeling allows for the detailed investigation of estradiol's metabolic fate and biotransformation pathways in research settings.
• Method Validation & GLP Compliance: Provides a stable, non-interfering standard essential for meeting rigorous Good Laboratory Practice (GLP) requirements in regulated bioanalytical laboratories.
• Environmental & Food Safety Testing: Applied in trace analysis for detecting estrogenic compounds in environmental samples and food products.

Basic Information

Product Name	17β-Estradiol-[2,3,4-13C3]
CAS No.	1261254-48-1
Molecular Formula	C18H2413C3O2
Molecular Weight	275.36 g/mol (for 13C3 labeled)
Synonyms	Estradiol-2,3,4-13C3; [2,3,4-13C3]-17β-Estradiol; 1,3,5(10)-Estratrien-3,17β-diol-2,3,4-13C3; E2-13C3; 17β-Estradiol-13C3; Isotope-labeled Estradiol; 13C3-17β-Estradiol; Stable Isotope-Labeled Internal Standard (SIL-IS) for Estradiol
EINECS	Contact for details

Quality Control

Every batch of 17β-Estradiol-[2,3,4-13C3] is manufactured and analyzed under strict quality management systems. We guarantee high chemical and isotopic purity, with comprehensive characterization using NMR, HPLC, and mass spectrometry to confirm structure, enrichment, and absence of unlabeled species. Certificates of Analysis (COA) detailing batch-specific results for identity, purity (HPLC), isotopic enrichment, and residual solvents are provided and traceable to internally certified reference standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere for long-term stability. This material is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to theoretical mass
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 99 atom % 13C
Chemical Purity (by 1H-NMR)	Conforms
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.