Description

1,25-Dihydroxyvitamin D2-[D3] is a high-purity, deuterated analog of the biologically active form of vitamin D, specifically designed for research and analytical applications. This compound serves as a critical internal standard in mass spectrometry, enabling precise quantification of vitamin D metabolites in complex biological matrices. It is essential for pharmaceutical R&D, clinical diagnostics, and nutritional science laboratories focused on studying vitamin D metabolism, deficiency, and related therapeutic interventions.

Application

• Mass Spectrometry Internal Standard: Primary use as a deuterated internal standard for the accurate LC-MS/MS analysis of 1,25-dihydroxyvitamin D2 and D3 in serum, plasma, and tissue samples.
• Clinical Research & Diagnostics: Enables reliable measurement of vitamin D status in studies related to bone health, autoimmune diseases, and cancer research.
• Pharmaceutical Development: Used in pharmacokinetic and metabolic studies for the development of new vitamin D-based therapeutics and analogs.
• Reference Standard: Serves as a certified reference material (CRM) for method validation and quality control in analytical laboratories.
• Nutritional Science: Facilitates research into vitamin D bioavailability, fortification, and its role in human health.
• Endocrinology Studies: Critical tool for investigating the role of the vitamin D endocrine system in calcium homeostasis and cell regulation.

Basic Information

Product Name	1,25-Dihydroxyvitamin D2-[D3]
CAS No.	1261254-46-9
Molecular Formula	C₂₈H₄₂D₃O₃
Molecular Weight	425.66 g/mol
Synonyms	Ergocalciferol-d3, 1,25-Dihydroxy; 1α,25-Dihydroxyvitamin D2-d3; 1,25-Dihydroxyergocalciferol-d3; (5Z,7E,22E)-(1S,3R)-1,3-Dihydroxy-9,10-secoergosta-5,7,10(19),22-tetraene-26,26,26-d3; Calcitriol-d3 (D2 analog); 1,25(OH)₂D2-d3; Vitamin D2 Hormone-d3
EINECS	Contact for details

Quality Control

Our 1,25-Dihydroxyvitamin D2-[D3] is manufactured under strict quality systems to ensure the highest standards of purity and isotopic enrichment required for analytical applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing results from HPLC, NMR, and MS analyses. We support compliance with ISO 17034 for reference material producers, and our quality protocols are designed to meet the rigorous demands of clinical and pharmaceutical research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC-MS)	Conforms
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (MS)	≥ 98 atom % D
Chemical Purity (NMR)	Conforms
Solubility	Soluble in ethanol, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.