Description

Biotin-Peg4-S-S-Nhs CAS NO 1260247-51-5 is a heterobifunctional crosslinker featuring a biotin moiety, a cleavable disulfide (S-S) bridge, and an amine-reactive NHS ester. This compound is engineered for the controlled conjugation and subsequent release of biomolecules, enabling advanced bioconjugation strategies in research and development. It is a critical reagent for scientists and manufacturers in the fields of proteomics, drug discovery, and diagnostic assay development who require precise, reversible labeling and purification of target proteins and other amine-containing compounds.

Application

• Controllable Biotinylation: For reversible labeling of antibodies, peptides, or other proteins via their primary amines, allowing for subsequent release under reducing conditions.
• Affinity Purification & Pull-Down Assays: Enables the capture of biotinylated targets on streptavidin/avidin resins, followed by gentle elution using agents like DTT or TCEP to break the disulfide bond.
• Drug Delivery & Bioconjugate Development: Serves as a cleavable linker in the construction of antibody-drug conjugates (ADCs) or other targeted therapeutic constructs.
• Surface Functionalization: Used to create cleavable biotin layers on biosensors, microarrays, or chip surfaces for studying biomolecular interactions.
• Cell Surface Labeling: For temporary tagging of cell surface proteins for isolation and analysis.
• Proteomic Sample Preparation: Facilitates the isolation and subsequent release of specific protein complexes or post-translationally modified proteins for mass spectrometry analysis.

Basic Information

Product Name	Biotin-Peg4-S-S-Nhs
CAS No.	1260247-51-5
Molecular Formula	C₃₃H₅₆N₄O₁₂S₂
Molecular Weight	764.95 g/mol
Synonyms	Biotin-PEG4-SS-NHS ester; Biotin-PEG4-disulfide-NHS; NHS-PEG4-SS-Biotin; 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(17-oxo-6,9,12,15-tetraoxa-3,18-dithia-16-azahenicosan-21-yl)pentanamide; Biotin-SS-PEG4-NHS; Biotinylated cleavable linker; EZ-Link™ Biotin-PEG4-SS-NHS (trade name analog); Succinimidyl-[(N-biotinamido-3,6,9,12-tetraoxapentadec-1-yl)-3,3'-dithiodipropionate]
EINECS	Contact for details

Quality Control

Our Biotin-Peg4-S-S-Nhs is manufactured under strict quality protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure identity, potency, and consistency. Certificates of Analysis (COA) with detailed specifications are available upon request to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and the NHS ester is susceptible to hydrolysis. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥95%
Water Content (KF)	≤1.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.