Description

Spdp-Peg8-Nhs Ester is a heterobifunctional crosslinking reagent featuring an amine-reactive NHS ester and a sulfhydryl-reactive pyridyldithiol group, connected by a flexible, hydrophilic PEG8 spacer. This molecular architecture is critical for creating stable, site-specific conjugates between proteins, peptides, and other biomolecules while maintaining solubility and reducing steric hindrance. It is an essential tool for researchers and developers in bioconjugation, antibody-drug conjugate (ADC) development, and diagnostic assay manufacturing, where controlled and efficient linkage is paramount.

Application

• Antibody-Drug Conjugate (ADC) Linker: Serves as a key chemical bridge for attaching cytotoxic payloads to monoclonal antibodies via cysteine residues.
• Protein-Protein/Peptide Conjugation: Enables the controlled crosslinking of different biomolecules for creating bispecific constructs or protein complexes.
• Surface Functionalization: Used to modify nanoparticles, biosensors, or chromatographic media with thiol-containing ligands for targeted binding.
• Diagnostic Reagent Preparation: Facilitates the labeling of antibodies, enzymes, or other proteins with reporter molecules (e.g., fluorophores, enzymes) for immunoassays.
• Polymer Modification: Incorporates functional thiol or amine groups into polymer backbones to create advanced biomaterials.
• Stability-Enhancing Conjugates: Used to PEGylate proteins at specific sites to improve pharmacokinetic profiles and reduce immunogenicity.

Basic Information

Product Name	Spdp-Peg8-Nhs Ester
CAS No.	1252257-56-9
Molecular Formula	C₃₈H₆₀N₄O₁₆S₂
Molecular Weight	904.99 g/mol
Synonyms	SPDP-PEG8-NHS Ester; NHS-PEG8-SPDP; Succinimidyl-[(N-maleimidopropionamido)-octaethyleneglycol] ester; N-Succinimidyl 3-((2-(2-(3-(2-pyridyldithio)propanamido)ethoxy)ethoxy)ethyl)propanamido)propanoate; Maleimide-PEG8-NHS Ester (SPDP derivative); 3-(2-Pyridyldithio)propionyl-PEG8-NHS; Heterobifunctional PEG Crosslinker, NHS-PEG8-PDP; SMPDP-PEG8-NHS
EINECS	Contact for details

Quality Control

Our Spdp-Peg8-Nhs Ester is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive bioconjugation applications. Each lot is characterized by HPLC, NMR, and mass spectrometry to confirm identity, purity, and functional integrity. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are provided with every shipment. We support development under GMP-grade conditions upon request.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay (Functional NHS Ester)	≥ 90%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.