Description

1-Oleoyl-2-{6-[4-(Dipyrrometheneboron Difluoride)Butanoyl]Amino}Hexanoyl-Sn-Glycero-3-Phosphoinositol-4,5-Bisphosphate (Ammonium Salt) is a highly specialized, fluorescently labeled phosphoinositide derivative designed for advanced biochemical research. This compound is critical for real-time visualization and quantitative analysis of key cellular signaling pathways involving phosphatidylinositol 4,5-bisphosphate (PIP2). It is an essential tool for researchers in cell biology, neuroscience, and pharmacology studying membrane dynamics, lipid-protein interactions, and intracellular trafficking.

Application

• Live-Cell Imaging Probes: Used as a fluorescent tracer to visualize PIP2 localization and dynamics in real-time within living cells using fluorescence microscopy.
• Lipid Kinase/Phosphatase Assays: Serves as a sensitive fluorescent substrate for in vitro enzymatic assays to study the activity of phosphoinositide kinases and phosphatases.
• Membrane Biophysics Research: Employed in model membrane systems (e.g., liposomes, supported lipid bilayers) to investigate lipid-lipid and lipid-protein interactions.
• Drug Discovery Screening: Utilized in high-throughput screening (HTS) platforms to identify novel modulators of phosphoinositide signaling pathways.
• Protein-Lipid Binding Studies: Acts as a probe in techniques like fluorescence polarization (FP) or surface plasmon resonance (SPR) to characterize the binding affinity of proteins to PIP2.
• Intracellular Signaling Studies: Applied in research focused on G-protein coupled receptor (GPCR) signaling, cytoskeletal reorganization, and ion channel regulation where PIP2 plays a central role.

Basic Information

Product Name	1-Oleoyl-2-{6-[4-(Dipyrrometheneboron Difluoride)Butanoyl]Amino}Hexanoyl-Sn-Glycero-3-Phosphoinositol-4,5-Bisphosphate (Ammonium Salt)
CAS No.	1246355-57-6
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	PIP2-BODIPY FL C5; Fluorescent PIP2; BODIPY® FL C5-PtdIns(4,5)P2; 1-Oleoyl-2-{6-[(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Propionyl)Amino]Hexanoyl}-Sn-Glycero-3-Phosphoinositol-4,5-Bisphosphate Ammonium Salt; BODIPY® FL Phosphatidylinositol 4,5-Bisphosphate; BODIPY® FL PI(4,5)P2; Fluorescent Phosphatidylinositol 4,5-Bisphosphate; Ammonium 1-oleoyl-2-{6-[4-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacen-3-yl)butanamido]hexanoyl}-sn-glycero-3-phospho-(1D-myo-inositol 4,5-bisphosphate)
EINECS	Contact for details

Quality Control

This high-purity biochemical is manufactured under strict quality control protocols to ensure batch-to-batch consistency and performance in sensitive applications. Each lot is characterized using advanced analytical techniques including HPLC, mass spectrometry, and NMR to confirm identity, purity, and labeling efficiency. Certificates of Analysis (COA) detailing spectral properties, purity, and functional performance data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature before opening to minimize moisture condensation. For long-term storage, solutions should be prepared in anhydrous solvents and stored under inert atmosphere.

Specification

Item	Specification
Appearance	Orange to red solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Excitation/Emission Maxima	~503 nm / ~512 nm
Solubility	Soluble in chloroform, methanol, DMSO
Functional Activity	Validated in lipid-binding or enzymatic assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.