Description

Biotin-Peg3-Sh CAS NO 1244028-52-1 is a high-purity, heterobifunctional polyethylene glycol (PEG) crosslinker featuring a biotin moiety and a thiol-reactive group. This compound is essential for creating stable, site-specific bioconjugates, enabling advanced research and development in targeted drug delivery and diagnostic systems. It is primarily utilized by pharmaceutical R&D teams, diagnostic kit manufacturers, and academic researchers in biochemistry and molecular biology.

Application

• Antibody-Drug Conjugate (ADC) Development: For the precise conjugation of cytotoxic payloads to monoclonal antibodies via thiol groups.
• Diagnostic Probe Labeling: Functionalization of proteins, peptides, or nanoparticles with biotin for detection in ELISA, lateral flow assays, or biosensors.
• Surface Functionalization: Immobilization of biomolecules onto gold surfaces or maleimide-activated chips for SPR (Surface Plasmon Resonance) studies.
• Affinity Chromatography Ligand Synthesis: Creating biotinylated capture ligands for streptavidin/avidin-based purification systems.
• Protein and Peptide Modification: Site-specific PEGylation to enhance solubility, stability, and pharmacokinetic properties of therapeutic biomolecules.
• Nanoparticle Targeting: Conjugation to liposomes or polymeric nanoparticles for targeted delivery applications.

Basic Information

Product Name	Biotin-Peg3-Sh
CAS No.	1244028-52-1
Molecular Formula	C23H41N5O7S2
Molecular Weight	563.73 g/mol
Synonyms	Biotin-PEG3-thiol; Biotin-PEG3-Maleimide (precursor); Biotinylated Triethylene Glycol Thiol; 2-[2-[2-[2-[5-[(3aS,4S,6aR)-2-Oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethoxy]ethoxy]ethoxy]ethyl 3-[(2,5-Dioxopyrrol-1-yl)oxy]propanoate (related); Biotin-PEG3-SH; Biotin-PEG3-C2-SH; EZ-Link Biotin-PEG3-Thiol
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Each batch of Biotin-Peg3-Sh is characterized by HPLC, NMR, and mass spectrometry to confirm identity, purity, and functional integrity. Certificates of Analysis (COA) with detailed chromatographic data are available upon request. We adhere to cGMP guidelines for the production of materials intended for research and pre-clinical development.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below under an inert atmosphere (argon or nitrogen) to prevent oxidation and hydrolysis. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Group Assay	≥ 90% (Thiol/Biotin)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.