Description

Pefloxacin-D5 is a deuterated analog of the broad-spectrum fluoroquinolone antibiotic, Pefloxacin, where five hydrogen atoms are replaced with deuterium. This stable isotope-labeled compound is an essential tool for quantitative bioanalytical research, providing a critical internal standard for mass spectrometry. It is primarily used by pharmaceutical R&D laboratories, contract research organizations (CROs), and analytical chemists to ensure accurate pharmacokinetic and metabolic studies, supporting drug development and regulatory compliance.

Application

• Internal Standard for LC-MS/MS Analysis: Serves as a critical reference compound for the precise quantification of Pefloxacin in biological matrices such as plasma, serum, and urine.
• Drug Metabolism and Pharmacokinetics (DMPK) Studies: Enables accurate tracking of absorption, distribution, metabolism, and excretion (ADME) profiles of the parent drug.
• Bioanalytical Method Development and Validation: Used to develop robust, sensitive, and specific analytical methods compliant with FDA and ICH guidelines.
• Clinical Research and Therapeutic Drug Monitoring (TDM): Facilitates reliable measurement of drug concentrations in patient samples to optimize dosing regimens.
• Pharmaceutical Impurity Profiling: Aids in the identification and quantification of related substances and degradation products in drug substances and products.
• Forensic and Anti-Doping Analysis: Supports accurate detection and confirmation of Pefloxacin in forensic toxicology and sports doping control.

Basic Information

Product Name	Pefloxacin-D5
CAS No.	1228182-51-1
Molecular Formula	C17H15D5FN3O3
Molecular Weight	339.36 g/mol (for C17H15D5FN3O3)
Synonyms	1-Ethyl-6-fluoro-1,4-dihydro-7-(4-methyl-1-piperazinyl)-4-oxo-3-quinolinecarboxylic Acid-d5; Pefloxacin (ethyl-d5); Pefloxacin-d5 (ethyl-d5); 1-Ethyl-d5-6-fluoro-1,4-dihydro-7-(4-methylpiperazin-1-yl)-4-oxoquinoline-3-carboxylic Acid; Pefloxacin (ethyl-2H5); Deuterated Pefloxacin
EINECS	Contact for details

Quality Control

Every batch of Pefloxacin-D5 is manufactured and analyzed under strict quality management systems. We guarantee high chemical and isotopic purity to meet the exacting demands of quantitative analytical applications. Comprehensive testing includes assay, isotopic enrichment (D-content), and identification of chemical and isotopic impurities via advanced techniques like HPLC, NMR, and Mass Spectrometry. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming compliance with specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-content)	≥ 98 atom % D
Chemical Purity (by HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.