Description

o-(3-Carboxypropyl)-O'-[2-(3-Mercaptopropionylamino)Ethyl]-Polyethylene Glycol Mw 5000 is a heterobifunctional polyethylene glycol (PEG) derivative engineered for advanced bioconjugation and surface modification. This compound matters because it provides a versatile linker with a carboxylic acid group for amine coupling and a thiol-reactive maleimide precursor (via the mercaptopropionylamino group) for site-specific protein and peptide modification. It is essential for researchers and manufacturers in pharmaceutical development, diagnostics, and materials science who require precise, stable PEGylation to enhance the solubility, stability, and pharmacokinetic profiles of therapeutic agents.

Application

• Bioconjugation and PEGylation Linker: Site-specific conjugation of peptides, proteins, antibodies, and oligonucleotides to improve therapeutic properties.
• Drug Delivery System Development: Creating functionalized surfaces on liposomes, nanoparticles, and micelles for targeted drug delivery.
• Diagnostic Reagent Preparation: Modifying enzymes, fluorescent dyes, or other detection molecules for use in immunoassays and biosensors.
• Surface Functionalization: Grafting onto materials to create non-fouling, biocompatible surfaces for medical devices and implants.
• Crosslinking Agent: For forming hydrogels or crosslinked polymer networks with controlled chemistry.
• Intermediate for Further Synthesis: Serving as a key building block for synthesizing more complex multi-arm or branched PEG derivatives.

Basic Information

Item	Detail
Product Name	o-(3-Carboxypropyl)-O'-[2-(3-Mercaptopropionylamino)Ethyl]-Polyethylene Glycol Mw 5000
CAS No.	1220112-75-3
Molecular Formula	CnH2n+2On+2 (PEG backbone) with functional end groups
Molecular Weight	~5000 Da (Average Molecular Weight)
Synonyms	Carboxy-PEG-Maleimide Precursor (MW 5000); α-Carboxy-ω-(2-(3-Mercaptopropionamido)ethyl) PEG; HOOC-PEG-SH Precursor; 3-Carboxypropyl-PEG5000-SPA; Maleimide-PEG-Acid (via activation); Heterobifunctional PEG Acid/Thiol; PEG5k-COOH/ Maleimide活性酯前体; Acido-PEG-tiol precursor
EINECS	Contact for details

Quality Control

Our o-(3-Carboxypropyl)-O'-[2-(3-Mercaptopropionylamino)Ethyl]-Polyethylene Glycol Mw 5000 is produced under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity, consistent molecular weight distribution, and functional group integrity. Certificates of Analysis (COA) are provided with each shipment, detailing specifications for critical parameters. We support development under cGMP guidelines where applicable.

Storage

Preserve in a tightly closed container, protected from light. Due to its hygroscopic (moisture-sensitive) and easily oxidized nature, store the product under an inert atmosphere (e.g., nitrogen or argon) at -20°C or below for long-term stability. For short-term use, store desiccated at 2-8°C. Allow the sealed container to reach room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white waxy solid or powder
Identification (IR)	Conforms to structure
Average Molecular Weight (GPC)	5000 ± 500 Da
Polydispersity Index (PDI)	≤ 1.05
Functional Group Substitution	> 95%
Assay (HPLC)	> 95% (PEG related substance)
Water Content (KF)	< 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.