Description

Arachidonic Acid-Biotinamide is a high-purity, chemically modified derivative of arachidonic acid, a vital polyunsaturated ω-6 fatty acid, conjugated with biotin. This specialized compound is engineered to leverage the biological activity of arachidonic acid with the high-affinity binding properties of biotin, enabling advanced biochemical research and diagnostic applications. It is an essential tool for researchers and developers in the life sciences, pharmaceutical, and nutraceutical industries who require precise molecular probes for studying lipid signaling pathways, inflammation, and cellular metabolism.

Application

• Biochemical Research Probe: Used as a molecular probe for studying arachidonic acid metabolism, eicosanoid signaling pathways, and lipid-protein interactions via biotin-streptavidin binding assays.
• Diagnostic Assay Development: Serves as a critical component in the development of ELISA, lateral flow assays, and other immunoassays for detecting lipid-related biomarkers.
• Affinity Purification & Pull-Down Assays: Facilitates the isolation and purification of arachidonic acid-binding proteins and receptors from complex biological samples.
• Cell Biology & Imaging: Employed in fluorescence microscopy and flow cytometry for visualizing lipid localization and trafficking within cells when paired with fluorescently labeled streptavidin.
• Pharmaceutical Screening: Used in high-throughput screening (HTS) platforms to identify novel drug candidates that modulate enzymes in the arachidonic acid cascade, such as cyclooxygenases (COX) and lipoxygenases (LOX).
• Nutraceutical & Lipidomics Research: Acts as a standard or tracer in mass spectrometry-based lipidomics to quantify and profile complex lipid species in biological systems.

Basic Information

Product Name	Arachidonic Acid-Biotinamide
CAS No.	1217901-28-4
Molecular Formula	C30H48N2O4S
Molecular Weight	532.78 g/mol
Synonyms	Biotinylated Arachidonic Acid; Arachidonoyl Biotin; 5Z,8Z,11Z,14Z-Eicosatetraenoyl Biotinamide; AA-Biotin; Biotin-AA; Biotinamidoarachidonic Acid; N-[(5Z,8Z,11Z,14Z)-Eicosa-5,8,11,14-tetraenoyl]biotinamide; Vitamin H-Arachidonate Conjugate
EINECS	Contact for details

Quality Control

Every batch of Arachidonic Acid-Biotinamide is manufactured under strict quality management systems and undergoes comprehensive analytical testing to ensure identity, purity, and consistency. We adhere to high-purity research-grade standards, with specifications typically meeting or exceeding 95% purity by HPLC. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting key parameters including assay, identity confirmation, and impurity profiles. Our quality commitment ensures reliable performance for your most sensitive research and development applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store unopened vials at -20°C or below under an inert atmosphere (e.g., argon or nitrogen). This product is easily oxidized (store under inert atmosphere) and light-sensitive (store away from light). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. Once opened, purge the container with an inert gas and reseal tightly.

Specification

Item	Specification
Appearance	White to off-white solid or oily solid
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 95.0%
Related Substances (HPLC)	Total impurities ≤ 5.0%
Solubility	Soluble in DMSO, DMF, ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.