Description

2-{N2-[N6-(4-Azido-2,3,5,6-Tetrafluorobenzoyl)-6-Aminocaproyl]-N6-(6-Biotinamidocaproyl)-L-Lysinylamido}] Ethyl 2-Carboxyethyl Disulfide is a sophisticated, heterobifunctional crosslinking reagent designed for advanced bioconjugation and proteomics research. This compound integrates a cleavable disulfide bridge with a photoactivatable aryl azide and a biotin affinity tag, enabling controlled, light-induced crosslinking followed by efficient purification or detection. It is an essential tool for researchers in pharmaceutical development and life sciences who require precise, reversible linkage strategies for studying protein-protein interactions, cell surface labeling, and affinity-based target isolation.

Application

• Photoaffinity Labeling (PAL): The aryl azide group allows for UV light-induced covalent crosslinking to target biomolecules, ideal for capturing transient or weak interactions in complex biological systems.
• Affinity Purification & Pull-Down Assays: The biotin moiety facilitates robust capture and isolation of crosslinked complexes using streptavidin-coated beads, enabling downstream analysis like mass spectrometry.
• Reversible Bioconjugation: The disulfide bond provides a cleavable linkage, allowing for the controlled release of captured targets under mild reducing conditions for further characterization.
• Proteomics & Interactome Mapping: Critical for identifying and validating protein-protein interaction networks, signaling pathways, and drug target engagement in living cells.
• Cell Surface Engineering & Labeling: Used to tag and study membrane proteins, receptors, and other extracellular targets while maintaining the option for subsequent elution.
• Development of Antibody-Drug Conjugates (ADCs) & Biotherapeutics: Serves as a model or research tool for designing next-generation therapeutic conjugates with cleavable linkers.
• Chemical Biology Probe Development: Enables the creation of activity-based probes for functional proteomics and target discovery campaigns.

Basic Information

Product Name	2-{N2-[N6-(4-Azido-2,3,5,6-Tetrafluorobenzoyl)-6-Aminocaproyl]-N6-(6-Biotinamidocaproyl)-L-Lysinylamido}] Ethyl 2-Carboxyethyl Disulfide
CAS No.	1217773-33-5
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Biotin-PEG3-SS-Photoactivatable Crosslinker; Heterobifunctional Biotin-Azide Crosslinker with Disulfide Bond; Cleavable Biotinylated Photoaffinity Label; 4-Azido-2,3,5,6-tetrafluorobenzoyl-LC-Biotin SS Crosslinker; Biotin-(AC5)-SS-(AC6)-Aryl Azide; Photo-cleavable Biotin Labeling Reagent; Biotin-PEG3-SS-Azide (Tetrafluorophenyl); N2-[N6-(4-Azido-2,3,5,6-tetrafluorobenzoyl)-6-aminocaproyl]-N6-(6-biotinamidocaproyl)-L-lysinylamido] ethyl 2-carboxyethyl disulfide
EINECS	Contact for details

Quality Control

Our production of this high-purity biochemical reagent adheres to stringent quality protocols. Each batch is characterized and tested to ensure identity, purity, and functional performance, with analysis typically including HPLC, mass spectrometry, and NMR. A comprehensive Certificate of Analysis (COA) detailing lot-specific results is provided to support your research reproducibility and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, keep under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Activity	Validated by crosslinking assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.