Description

2-[2-[2-[2-[6-(Biotinylaminohexanoyl]Aminoethoxy]Ethoxy]Ethoxy]-4-[3-(Trifluoromethyl)-3H-Diazirin-3-Yl]Benzoic Acid n-Hydroxysuccinimide Ester is a sophisticated heterobifunctional crosslinking reagent designed for advanced bioconjugation and photoaffinity labeling applications. This compound integrates a biotin moiety for streptavidin-based capture and a photoreactive diazirine group for covalent bond formation upon UV light activation, enabling the study of protein-protein interactions and biomolecular localization. It is an essential tool for researchers in proteomics, chemical biology, and drug discovery who require high-purity, reliable reagents for labeling and pull-down assays.

Application

• Photoaffinity Labeling (PAL): Covalent tagging of biomolecules and their binding partners for interaction mapping.
• Chemical Proteomics: Identification and characterization of protein targets and off-targets in drug discovery.
• Biotin-Streptavidin Pull-Down Assays: Isolation and purification of labeled protein complexes for mass spectrometry analysis.
• Membrane Protein Studies: Crosslinking and labeling of integral membrane proteins and their interactors.
• Activity-Based Protein Profiling (ABPP): Profiling enzyme activities within complex biological samples.
• Development of Diagnostic Reagents: Creating sensitive detection probes for immunoassays and biosensors.
• Site-Specific Bioconjugation: Controlled attachment of probes, drugs, or other molecules to proteins, antibodies, or nanoparticles.

Basic Information

Product Name	2-[2-[2-[2-[6-(Biotinylaminohexanoyl]Aminoethoxy]Ethoxy]Ethoxy]-4-[3-(Trifluoromethyl)-3H-Diazirin-3-Yl]Benzoic Acid n-Hydroxysuccinimide Ester
CAS No.	1217722-17-2
Molecular Formula	C35H46F3N6O9S
Molecular Weight	798.85 g/mol
Synonyms	Biotin-PEG4-Photo-Leucine-NHS Ester; Biotinylated Photo-Leucine NHS Ester; Biotin-PEG4-Phe(3-(trifluoromethyl)-3H-diazirin-3-yl)-NHS; NHS-PEG4-Biotin-Photo-Leucine; Photoactivatable Biotin Crosslinker; Biotin Photoprobe NHS Ester; Biotin-(PEG)4-3-[3-(Trifluoromethyl)diazirin-3-yl]phenylalanine NHS ester; Heterobifunctional Biotin-Diazirine Crosslinker
EINECS	Contact for details

Quality Control

Every batch of this high-value biochemical reagent is manufactured and tested under strict quality control protocols to ensure identity, purity, and functional performance. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing key parameters such as assay purity, functional group activity, and absence of critical impurities. Our production adheres to cGMP principles where applicable, ensuring consistency and reliability for critical research and development applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store desiccated under an inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay (Functional NHS Ester)	≥ 90%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.