Description

2-[2-[2-[2-[6-(Biotinylaminohexanoyl]Aminoethoxy]Ethoxy]Ethoxy]-4-[3-(Trifluoromethyl)-3H-Diazirin-3-Yl]Benzoic Acid, Methyl Ester is a sophisticated bifunctional chemical probe designed for advanced photoaffinity labeling and bioconjugation studies. This compound integrates a biotin tag for streptavidin-based capture and a photoreactive diazirine group for covalent cross-linking upon UV irradiation, enabling the identification and isolation of biomolecular interaction partners. It is an essential tool for researchers in proteomics, chemical biology, and drug discovery who require high-purity, reliable reagents for mapping protein-protein interactions and studying complex biological systems.

Application

• Photoaffinity Labeling (PAL): Covalent tagging of proteins and other biomolecules for interaction mapping and target identification.
• Chemical Proteomics: Identification of drug targets and off-targets by enriching and analyzing probe-labeled proteins.
• Bioconjugation & Immobilization: Site-specific modification of surfaces, nanoparticles, or other molecules for assay development and biosensor fabrication.
• Activity-Based Protein Profiling (ABPP): Functional characterization of enzymes and discovery of active sites within complex proteomes.
• Structural Biology Studies: Probing ligand-binding sites and protein conformations to aid in structure-based drug design.
• Diagnostic Reagent Development: Creating sensitive detection systems by leveraging the high-affinity biotin-streptavidin interaction.

Basic Information

Product Name	2-[2-[2-[2-[6-(Biotinylaminohexanoyl]Aminoethoxy]Ethoxy]Ethoxy]-4-[3-(Trifluoromethyl)-3H-Diazirin-3-Yl]Benzoic Acid, Methyl Ester
CAS No.	1217691-71-8
Molecular Formula	C34H48F3N5O8S
Molecular Weight	743.84 g/mol
Synonyms	Biotin-PEG4-Diazirine Methyl Ester; Biotinylated Photoaffinity Probe; Biotin-PEG4-TFD Methyl Ester; Methyl 4-[3-(trifluoromethyl)diazirin-3-yl]-2-[2-[2-[2-[6-(biotinylamino)hexanoylamino]ethoxy]ethoxy]ethoxy]benzoate; Biotin-PEG4-Photo-Leucine Analogue; TFD-Biotin Methyl Ester; Photoactivatable Biotin Reagent; Biotin Photoprobe
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Every batch of this high-value biochemical probe is characterized by HPLC, NMR, and mass spectrometry to confirm identity, purity, and functional integrity. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing all critical parameters. Our manufacturing and quality systems adhere to ISO 9001 principles, ensuring traceability and consistency for research and development applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is strictly light-sensitive (protect from all light exposure) and hygroscopic (moisture-sensitive). For long-term stability, store under inert atmosphere (argon or nitrogen) and allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.