Description

25-Hydroxyvitamin D2-[D3] is a high-purity, deuterium-labeled internal standard critical for accurate quantitative analysis in mass spectrometry. This compound is essential for researchers and quality control laboratories requiring precise measurement of vitamin D metabolites in complex biological matrices. It is primarily used in clinical research, pharmaceutical development, and nutritional science for method validation and ensuring data integrity.

Application

• Internal Standard for LC-MS/MS: Used as a deuterated internal standard for the precise quantification of 25-hydroxyvitamin D2 and D3 in serum, plasma, and other biological samples.
• Clinical Diagnostics & Research: Enables accurate assessment of vitamin D status in studies related to bone health, immune function, and chronic diseases.
• Pharmaceutical Bioanalysis: Supports pharmacokinetic and pharmacodynamic studies for drug development involving vitamin D analogs or related pathways.
• Method Development & Validation: Critical for developing, optimizing, and validating robust analytical methods in accordance with guidelines like CLSI, FDA, and EMA.
• Food & Nutritional Supplement Analysis: Used to accurately determine vitamin D content and metabolite profiles in fortified foods and dietary supplements.
• Reference Material: Serves as a certified reference material (CRM) or high-grade standard for calibrating analytical instruments and ensuring laboratory proficiency.

Basic Information

Product Name	25-Hydroxyvitamin D2-[D3]
CAS No.	1217467-39-4
Molecular Formula	C28H43DO3
Molecular Weight	402.65 g/mol (for monoisotopic mass)
Synonyms	25-Hydroxyergocalciferol-d3; (3β,5Z,7E,22E)-9,10-Secoergosta-5,7,10(19),22-tetraen-3-ol-d3; 25-OH Vitamin D2-d3; 25-Hydroxyvitamin D2 d3; Deuterated 25-Hydroxyvitamin D2; 25(OH)D2-d3; Calcidiol-d3 (D2 form); Internal Standard for 25-OH-D2
EINECS	Contact for details

Quality Control

Every batch of 25-Hydroxyvitamin D2-[D3] is manufactured and analyzed under strict quality systems. We provide comprehensive Certificates of Analysis (COA) detailing purity, isotopic enrichment, and chromatographic data to ensure it meets the stringent requirements for use as an internal standard. Our quality commitment aligns with the needs of GMP/GLP-compliant laboratories and research facilities globally.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. Due to its hygroscopic and light-sensitive nature, allow the vial to equilibrate to room temperature before opening to minimize moisture uptake and degradation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC-MS)	Conforms
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 99.0 atom % D
Chemical Purity (by NMR)	Conforms
Solubility	Soluble in ethanol, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.