Description

Amiloride 15N3 is a stable isotope-labeled analog of the potassium-sparing diuretic amiloride, specifically enriched with three nitrogen-15 atoms. This high-purity reference material is essential for precise quantitative analysis in pharmaceutical research and development. It is primarily used by analytical chemists and researchers in the pharmaceutical and contract research organization (CRO) sectors for applications such as internal standard calibration in mass spectrometry.

Application

• Internal Standard for LC-MS/MS: Serves as a critical, non-interfering internal standard for the accurate quantification of amiloride and its metabolites in complex biological matrices like plasma, serum, and urine.
• Pharmacokinetic and Bioavailability Studies: Enables precise tracking and measurement of drug absorption, distribution, metabolism, and excretion (ADME) in clinical and pre-clinical research.
• Metabolite Identification and Profiling: Facilitates the identification and structural elucidation of drug metabolites using advanced spectroscopic techniques.
• Method Development and Validation: Used as a certified reference material to develop, optimize, and validate robust analytical methods in compliance with regulatory guidelines (e.g., FDA, EMA).
• Doping Control and Forensic Analysis: Employed in specialized testing laboratories for the definitive identification and quantification of diuretics.
• Stable Isotope Dilution Assays: Provides the highest accuracy in quantitative assays by correcting for sample preparation and instrumental variability.

Basic Information

Item	Details
Product Name	Amiloride 15N3
CAS No.	1217169-93-1
Molecular Formula	C6H8Cl2N7O • HCl (with three 15N atoms)
Molecular Weight	~302.1 (exact mass varies with isotopic enrichment)
Synonyms	Amiloride-15N3; 3,5-Diamino-N-(aminoiminomethyl)-6-chloropyrazinecarboxamide-15N3 hydrochloride; N-Amidino-3,5-diamino-6-chloropyrazinecarboxamide-15N3 hydrochloride; MK-870-15N3; Midamor-15N3
EINECS	Contact for details

Quality Control

Every batch of Amiloride 15N3 is manufactured and tested under strict quality management systems. We provide comprehensive analytical data to support its use as a certified reference standard, including confirmation of isotopic enrichment and chemical purity. A detailed Certificate of Analysis (COA) is supplied with each shipment, documenting results for identity, assay, isotopic purity, and related substances by HPLC and MS.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and must be kept in a desiccated environment to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (15N3)	≥ 98 atom % 15N
Chemical Purity (NMR)	Conforms
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.