Description

5-Methylcotinine-D3 is a deuterium-labeled isotopologue of 5-methylcotinine, a key metabolite of nicotine. This stable isotope-labeled compound is essential for use as an internal standard in quantitative analytical methods, ensuring high accuracy and precision in measurement. It is primarily utilized by research laboratories, contract research organizations (CROs), and pharmaceutical companies engaged in pharmacokinetic studies, tobacco biomarker research, and clinical diagnostics.

Application

• Internal Standard for LC-MS/MS Analysis: Critical for the accurate quantification of 5-methylcotinine and related alkaloids in complex biological matrices like plasma, urine, and saliva.
• Pharmacokinetic and Metabolic Studies: Used in research to track the absorption, distribution, metabolism, and excretion (ADME) of nicotine and its metabolites.
• Tobacco Exposure Biomarker Research: Enables precise measurement of tobacco consumption and exposure in epidemiological and clinical studies.
• Clinical Diagnostic Assay Development: Serves as a reference standard in the development and validation of diagnostic tests for smoking status and cessation monitoring.
• Forensic Toxicology: Applied in forensic analyses to provide definitive evidence of tobacco use with high specificity.
• Drug Development and Regulatory Submissions: Supports bioanalytical method validation for regulatory filings (e.g., with FDA, EMA) requiring stringent data integrity.

Basic Information

Product Name	5-Methylcotinine-D3
CAS No.	1217003-12-7
Molecular Formula	C10H10D3NO2
Molecular Weight	179.23 g/mol (approx.)
Synonyms	5-Methylcotinine-d3; (5S)-1-Methyl-5-(pyridin-3-yl)-2-pyrrolidinone-d3; 3-[(2S)-1-Methyl-5-oxo-2-pyrrolidinyl]pyridine-d3; 5-Methylcotinine (methyl-d3); Deuterated 5-Methylcotinine; Cotinine metabolite D3 standard; Nicotine metabolite D3 internal standard
EINECS	Contact for details

Quality Control

Every batch of 5-Methylcotinine-D3 is manufactured and analyzed under strict quality management systems. We provide comprehensive Certificates of Analysis (COA) that detail isotopic purity, chemical purity, and identity confirmation. Our quality commitment ensures the material meets the stringent requirements for use in regulated bioanalytical and clinical research, supporting data integrity for GLP and GMP environments.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-content)	≥ 98.0 atom % D
Chemical Purity (NMR)	Conforms
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.