Description

Cytosine-13C,15N2 is a stable isotope-labeled analog of the fundamental nucleobase cytosine, specifically enriched with carbon-13 and nitrogen-15 isotopes at designated positions. This compound is essential for advanced analytical research, providing an internal standard for precise quantification and mechanistic studies in mass spectrometry and NMR spectroscopy. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors for metabolomics, proteomics, and drug metabolism and pharmacokinetics (DMPK) studies.

Application

• Internal Standard for Mass Spectrometry (MS): Used as a quantitative reference standard in LC-MS and GC-MS analyses to ensure accuracy in measuring endogenous cytosine and related metabolites.
• Nuclear Magnetic Resonance (NMR) Spectroscopy: Serves as a crucial tracer in metabolic flux analysis and structural biology studies, allowing for the detailed tracking of biochemical pathways.
• Pharmaceutical Research & Development: Critical for drug discovery and development, particularly in studying the metabolism and distribution of nucleoside-based therapeutics and anticancer agents.
• Clinical Diagnostics & Metabolomics: Enables precise profiling of metabolic disorders by serving as a stable isotope-labeled standard in biomarker discovery and validation assays.
• Biochemical Pathway Tracing: Applied in cell culture and in vivo studies to elucidate the synthesis and catabolism of nucleotides and nucleic acids.
• Quality Control for Labeled Compounds: Used as a reference material to certify the purity and isotopic enrichment of other labeled nucleotides and nucleosides.

Basic Information

Product Name	Cytosine-13C,15N2
CAS No.	1216616-31-7
Molecular Formula	C413CH515N3O
Molecular Weight	114.07 g/mol (for labeled form)
Synonyms	4-Amino-2-hydroxypyrimidine-13C,15N2; 2-Oxo-4-aminopyrimidine-13C,15N2; 6-Aminopyrimidin-2(1H)-one-13C,15N2; [13C,15N2]-Cytosine; Cytosine (13C,15N2); Isotopically labeled cytosine; Stable isotope-labeled cytosine; 13C,15N2-Cytosine
EINECS	Contact for details

Quality Control

Our Cytosine-13C,15N2 is manufactured under strict quality systems to ensure the highest standards of isotopic and chemical purity. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing parameters such as isotopic enrichment (Atom % 13C and 15N), chemical purity by HPLC, and identification by spectroscopic methods. We support research and compliance needs in regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 99 atom % 13C; ≥ 99 atom % 15N
Chemical Purity	≥ 95% (by HPLC, area %)
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.