Description

Rac Cotinine-13C,D3 CAS NO 1215842-75-3 is a stable isotope-labeled internal standard, specifically a racemic mixture of cotinine where the molecule is enriched with carbon-13 and deuterium. This compound is critically important for ensuring the accuracy and precision of quantitative bioanalytical measurements in mass spectrometry. It is an essential tool for researchers and quality control laboratories in the pharmaceutical, clinical research, and forensic toxicology sectors who require definitive quantification of cotinine, a primary metabolite of nicotine, in complex biological matrices.

Application

• Quantitative Bioanalysis: Serves as an internal standard for the precise quantification of cotinine in plasma, serum, and urine via LC-MS/MS and GC-MS.
• Pharmacokinetic/Pharmacodynamic (PK/PD) Studies: Enables accurate tracking of nicotine metabolism and clearance rates in clinical trials for smoking cessation therapies.
• Forensic Toxicology: Used in standardized assays to confirm nicotine exposure levels with high reliability for legal and occupational testing.
• Biomarker Research: Facilitates studies on tobacco use and its correlation with disease by providing a stable reference point for cotinine measurement.
• Method Development & Validation: Critical for establishing and validating new analytical methods in compliance with regulatory guidelines (e.g., FDA, EMA).
• Environmental & Occupational Health Monitoring: Supports the analysis of cotinine as a biomarker for secondhand smoke exposure in environmental studies.

Basic Information

Product Name	Rac Cotinine-13C,D3
CAS No.	1215842-75-3
Molecular Formula	C10H913CD3N2O
Molecular Weight	180.22 g/mol (approximate)
Synonyms	(±)-Cotinine-13C,D3; DL-Cotinine-13C,D3; 5-(1-Methyl-2-pyrrolidinyl)-2-pyridinone-13C,D3; 1-Methyl-5-(3-pyridyl)-2-pyrrolidinone-13C,D3; Cotinine (methyl-d3, 2,4,5,6-13C4); Cotinine-d3-13C4; Nicotine metabolite cotinine-13C,D3; S-(–)-Cotinine-13C,D3 (racemic mixture)
EINECS	Contact for details

Quality Control

Every batch of Rac Cotinine-13C,D3 is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure identity, purity, and isotopic enrichment meet the highest standards. Our quality assurance protocol is designed to support GLP and GMP environments. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing results for assay, isotopic purity, chemical purity, and specific tests for residual solvents and impurities.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity	≥ 99 atom % 13C; ≥ 98 atom % D
Chemical Purity (NMR)	Conforms
Residual Solvents (GC)	Meets ICH guidelines
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.