Description

Propargyl-Nh-Peg3-C2-Nhs Ester is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring a propargyl group and an N-hydroxysuccinimide (NHS) ester. This compound is essential for creating stable, site-specific bioconjugates through orthogonal click chemistry and amine coupling reactions. It is primarily utilized by researchers and manufacturers in the fields of bioconjugation, proteomics, and advanced materials science for labeling, immobilization, and polymer modification.

Application

• Bioconjugation and Protein Labeling: The NHS ester reacts with primary amines on proteins or antibodies, while the propargyl group enables subsequent click chemistry (e.g., CuAAC) for attaching fluorescent dyes, drugs, or other functional probes.
• PEGylation and Linker Synthesis: Serves as a key building block for synthesizing PEG-based drug delivery systems, creating hydrophilic spacers to improve solubility and pharmacokinetics of therapeutic agents.
• Surface Functionalization: Used to modify surfaces (e.g., chips, nanoparticles, beads) with alkyne groups for the controlled immobilization of biomolecules via azide-alkyne cycloaddition.
• Polymer Chemistry: Acts as a functional monomer or crosslinking agent in the synthesis of smart polymers and hydrogels with tailored biological properties.
• Diagnostic Reagent Development: Employed in the fabrication of diagnostic assays and biosensors by linking recognition elements (e.g., antibodies, oligonucleotides) to signal-generating components.
• ADC (Antibody-Drug Conjugate) Research: Facilitates the development of novel ADC linkers, providing a stable bridge between the antibody and the cytotoxic payload.

Basic Information

Product Name	Propargyl-Nh-Peg3-C2-Nhs Ester
CAS No.	1214319-94-4
Molecular Formula	C19H29N3O8
Molecular Weight	427.45 g/mol
Synonyms	Propargyl-PEG3-NHS Ester; Propargyl-PEG3-C2-NHS Ester; 2,5-Dioxopyrrolidin-1-yl 3-[2-[2-(2-prop-2-ynoxyethoxy)ethoxy]ethoxy]propanoate; Alkyne-PEG3-NHS Ester; NHS-PEG3-Propargyl; Propargyl-PEG3-NHS; Propargylamino-PEG3-NHS Ester; N-(Propargyl)-3,6,9-trioxaundecanedioic acid imide
EINECS	Contact for details

Quality Control

Our Propargyl-Nh-Peg3-C2-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to ensure high purity and consistent performance for sensitive research and development applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and the NHS ester moiety is susceptible to hydrolysis. For long-term storage, keep under an inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC/MS)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.