Description

2-(Dimethylamino)-4-(2-Pyridinyldithio)Butanoic Acid 2,5-Dioxo-1-Pyrrolidinyl Ester is a specialized heterobifunctional crosslinking agent designed for the controlled conjugation of biomolecules. Its core value lies in its dual functionality, featuring an amine-reactive NHS ester and a cleavable disulfide bond, enabling the creation of reversible linkages critical for advanced bioconjugation strategies. This compound is essential for researchers and manufacturers in pharmaceutical development, proteomics, and diagnostic assay production, where precise and reversible protein modification is required.

Application

• Antibody-Drug Conjugate (ADC) Linker Development: Used to create cleavable linkages between cytotoxic payloads and monoclonal antibodies, facilitating targeted drug delivery.
• Protein-Protein Crosslinking: Enables the study of protein interactions and complex formation in structural biology and proteomics research.
• Immobilization of Biomolecules: For attaching peptides, proteins, or enzymes to solid supports (e.g., beads, biosensors, microarrays) via a reversible disulfide bond.
• Surface Functionalization: Modification of gold nanoparticles or other surfaces with pyridyl disulfide groups for thiol-specific coupling in nanomedicine.
• Controlled Release Systems: Designing drug delivery carriers where the therapeutic agent is released upon reduction of the disulfide bond in intracellular environments.
• Bioconjugation Reagent Kits: A key component in commercial and in-house kits for labeling antibodies, enzymes, or other proteins with fluorophores, haptens, or other molecules.

Basic Information

Item	Details
Product Name	2-(Dimethylamino)-4-(2-Pyridinyldithio)Butanoic Acid 2,5-Dioxo-1-Pyrrolidinyl Ester
CAS No.	1193111-73-7
Molecular Formula	C15H19N3O4S2
Molecular Weight	369.46 g/mol
Synonyms	N-Succinimidyl 3-(2-pyridyldithio)propionate (SPDP) derivative; 2-(Dimethylamino)-4-(pyridin-2-yldisulfanyl)butanoic acid 2,5-dioxopyrrolidin-1-yl ester; NHS-Pyridyldithio Propionate; Heterobifunctional Crosslinker (Amine and Sulfhydryl Reactive); Pyridyl Disulfide NHS Ester; PDSP-NHS; 3-(2-Pyridyldithio)propionyl-N-hydroxysuccinimide ester analog; N-(3-(2-Pyridyldithio)propionyloxy)succinimide derivative
EINECS	Contact for details

Quality Control

Our production of this high-purity crosslinking reagent adheres to stringent quality protocols. Every batch is characterized by HPLC, NMR, and mass spectrometry to confirm structural identity and purity. We provide comprehensive Certificates of Analysis (COA) detailing assay, impurity profiles, and functional performance data, ensuring reliability for critical research and cGMP-guided development workflows.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.