Description

Uridine 5'-(Tetrahydrogen Triphosphate), 2'-Deoxy-5-Ethynyl- Triethylamine is a chemically modified nucleoside triphosphate analog designed for advanced biochemical research and diagnostic applications. This compound is critical for studying nucleic acid metabolism, enzyme mechanisms, and for the development of novel molecular probes. It is primarily utilized by researchers and manufacturers in the fields of pharmaceutical development, molecular biology, and diagnostic assay production. Its unique ethynyl modification provides a versatile handle for bioorthogonal chemistry, enabling precise labeling and tracking of biomolecules.

Application

• Biochemical Research: As a substrate analog for studying DNA/RNA polymerases, reverse transcriptases, and other nucleic acid-processing enzymes.
• Probe Synthesis: Used in the enzymatic synthesis of labeled nucleotides for fluorescence in situ hybridization (FISH) and other detection techniques.
• Antiviral Drug Development: Serves as a potential building block or probe in the research of nucleoside analog-based antiviral therapies.
• Click Chemistry: The 5-ethynyl group enables copper-catalyzed azide-alkyne cycloaddition (CuAAC) for the site-specific conjugation of biomolecules.
• Diagnostic Kits: Incorporated into nucleotides for the manufacture of diagnostic assays that require highly specific labeling.
• Enzyme Kinetics: Employed in mechanistic studies to understand the inhibition or activity of viral and cellular polymerases.

Basic Information

Product Name	Uridine 5'-(Tetrahydrogen Triphosphate), 2'-Deoxy-5-Ethynyl- Triethylamine
CAS No.	1191275-46-3
Molecular Formula	C₁₄H₂₃N₄O₁₄P₃ (or C14H23N4O14P3)
Molecular Weight	612.28 g/mol
Synonyms	2'-Deoxy-5-ethynyluridine 5'-triphosphate triethylammonium salt; 5-Ethynyl-2'-deoxyuridine 5'-triphosphate; EdUTP; 5-Ethynyl-dUTP; 2'-Deoxy-5-ethynyluridine triphosphate; dEUTP; 5-Ethynyl-2'-deoxyuridine triphosphate triethylamine salt
EINECS	Contact for details

Quality Control

Our production of this high-purity biochemical adheres to stringent quality protocols. Every batch is characterized and tested to ensure identity, purity, and functional performance. A comprehensive Certificate of Analysis (COA) is provided, detailing results from advanced analytical techniques including HPLC, NMR, and mass spectrometry. We support research and GMP-grade manufacturing needs with reliable, batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This material is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption. For long-term storage, keep desiccated.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Triethylammonium Content	3.0 - 4.0 equivalents
Biological Activity	Suitable for enzymatic incorporation (tested)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.