Description

Biotin-Peg5-Azide is a high-purity, heterobifunctional crosslinking reagent designed for advanced bioconjugation. This compound is essential for creating stable, site-specific linkages between biomolecules, such as proteins and oligonucleotides, and various surfaces or detection probes. It is a critical tool for researchers and developers in pharmaceutical R&D, diagnostic assay development, and proteomics, enabling precise labeling and immobilization strategies.

Application

• ADC (Antibody-Drug Conjugate) Development: Site-specific conjugation of cytotoxic payloads to antibodies via click chemistry.
• Diagnostic Assay Fabrication: Immobilization of capture antibodies or antigens onto azide-functionalized microplates or biosensors.
• Protein Labeling & Profiling: Introduction of biotin tags onto proteins or peptides for purification or detection using streptavidin systems.
• Cell Surface Engineering: Metabolic labeling and subsequent click chemistry modification for studying cell membrane dynamics.
• Polymer & Material Science: Functionalization of nanoparticles, hydrogels, or other polymeric materials with bioactive ligands.
• Nucleic Acid Modification: Conjugation of oligonucleotides with biotin for detection or pull-down assays.

Basic Information

Product Name	Biotin-Peg5-Azide
CAS No.	1163732-89-5
Molecular Formula	C28H50N8O9S
Molecular Weight	674.81 g/mol
Synonyms	Biotin-PEG5-N3; Biotin-Pentaglycol-azide; 5-Azido-3,6,9,12,15-pentaoxa-24-thia-2-azaoctacosan-28-oic acid, 1-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl] ester; Biotinylated PEG Azide; N3-PEG5-Biotin; Azido-PEG5-Biotin; Biotin-PEG5-N3
EINECS	Contact for details

Quality Control

Every batch of Biotin-Peg5-Azide is manufactured under strict quality management systems and undergoes comprehensive analytical testing. We ensure high purity (typically >95% by HPLC) and identity confirmation via NMR and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting purity, assay, and impurity profiles to support your regulatory and research requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a dry environment before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.