Description

4-Methylumbelliferyl 2-Azido-2-Deoxy-3-o-(Tetra-o-Acetyl-β-D-Galactopyranosyl)-4,6-o-Phenylmethylene-α-D-Galactopyranoside is a specialized fluorogenic glycosidase substrate, critical for the sensitive detection and characterization of specific carbohydrate-active enzymes. Its value lies in its complex, tailored structure, which allows for precise biochemical research and diagnostic assay development. This compound is essential for researchers and manufacturers in glycobiology, enzymology, and pharmaceutical development, particularly for studying enzyme mechanisms and screening for inhibitors.

Application

• Glycosidase Enzyme Assays: As a fluorogenic substrate for the detection and kinetic analysis of specific α- and β-galactosidases and related enzymes.
• Biochemical Research: Used in mechanistic studies of carbohydrate-processing enzymes to understand substrate specificity and catalytic activity.
• Diagnostic Kit Development: A key component in the formulation of sensitive diagnostic kits for enzyme deficiency disorders, such as certain lysosomal storage diseases.
• High-Throughput Screening (HTS): Employed in pharmaceutical research to screen compound libraries for potential glycosidase inhibitors as drug candidates.
• Glycobiology Studies: A tool for investigating glycosylation pathways, glycan processing, and carbohydrate metabolism in cellular systems.
• Quality Control in Enzyme Production: Used to assay the activity and purity of commercially produced glycosidase enzymes.

Basic Information

Item	Detail
Product Name	4-Methylumbelliferyl 2-Azido-2-Deoxy-3-o-(Tetra-o-Acetyl-β-D-Galactopyranosyl)-4,6-o-Phenylmethylene-α-D-Galactopyranoside
CAS No.	1147438-61-6
Molecular Formula	C₃₇H₃₈N₃O₁₆
Molecular Weight	780.71 g/mol
Synonyms	4-MU-Gal-β-Az; 4-Methylumbelliferyl β-D-galactopyranoside derivative; 2-Azido-2-deoxy-3-O-(tetra-O-acetyl-β-D-galactopyranosyl)-4,6-O-phenylmethylene-α-D-galactopyranosyl 4-methylumbelliferone; Fluorogenic galactosidase substrate; Galactosidase substrate 1147438-61-6; 4-MU-β-D-Galactopyranoside azido derivative; 4-Methylumbelliferyl glycoside substrate
EINECS	Contact for details

Quality Control

Every batch of our 4-Methylumbelliferyl glycosidase substrate is produced and tested under strict quality management protocols to ensure the highest purity and batch-to-batch consistency required for sensitive research applications. We provide comprehensive Certificates of Analysis (COA) detailing purity, identity, and performance in standard assay conditions. Our quality systems are designed to meet the rigorous standards expected in pharmaceutical and life science research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is both light-sensitive (store away from light) and hygroscopic (moisture-sensitive). For long-term storage, consider under an inert atmosphere. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers
Functional Activity	≥ 90% of reference standard in standard galactosidase assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.