Description

4-Methylumbelliferyl 2-Azido-2-Deoxy-4,6-o-Phenylmethylene-α-D-Galactopyranoside is a specialized fluorogenic glycosidase substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise detection and quantification of specific glycosidase enzyme activity. It is primarily utilized in biochemical research, diagnostic kit development, and pharmaceutical screening for carbohydrate-active enzymes.

Application

• Biochemical Research: As a chromogenic/fluorogenic substrate for studying the kinetics and inhibition of specific α-galactosidases and related enzymes.
• Diagnostic Assay Development: Used in the formulation of enzyme-linked assays for the detection of genetic disorders like Fabry disease, which involves α-galactosidase A deficiency.
• High-Throughput Screening (HTS): Employed in pharmaceutical discovery platforms to identify potential glycosidase inhibitors or activators for therapeutic development.
• Enzyme Characterization: Essential for determining the specificity, activity, and purity of glycosidase enzymes during protein purification and functional analysis.
• Quality Control in Biomanufacturing: Used to monitor and validate the activity of enzyme-based products or recombinant proteins in biopharmaceutical production.

Basic Information

Product Name	4-Methylumbelliferyl 2-Azido-2-Deoxy-4,6-o-Phenylmethylene-α-D-Galactopyranoside
CAS No.	1147438-59-2
Molecular Formula	C23H21N3O8
Molecular Weight	467.43 g/mol
Synonyms	4-MU-2-Azido-2-deoxy-4,6-O-benzylidene-α-D-galactopyranoside; 4-Methylumbelliferyl 2-azido-2-deoxy-4,6-O-benzylidene-α-D-galactopyranoside; 2-Azido-2-deoxy-4,6-O-benzylidene-α-D-galactopyranosyl 4-methylumbelliferone; Fluorogenic α-galactosidase substrate; Galactosidase substrate 4-MU-α-Gal-2Az-Bn; 4-MU-α-Gal-2Az-Bn; CAS 1147438-59-2
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl 2-Azido-2-Deoxy-4,6-o-Phenylmethylene-α-D-Galactopyranoside is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard enzymatic assays. We adhere to relevant industry guidelines for biochemical reagents to support your compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. For long-term storage, consider desiccation to maintain stability. Allow the product to equilibrate to room temperature in a dry atmosphere before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Specific Rotation	Contact for details
Fluorogenic Activity	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.