Description

Ac-Val-Glu-His-Asp-Afc CAS NO 1135418-22-2 is a synthetic, fluorogenic peptide substrate specifically designed for the detection and quantification of caspase activity. This compound is a critical tool in biochemical research, enabling the study of apoptosis (programmed cell death) and related cellular pathways. It is primarily utilized by researchers and scientists in the fields of oncology, immunology, and drug discovery for high-throughput screening and mechanistic studies.

Application

• Caspase Activity Assays: Specifically designed as a substrate for caspase-3 and related caspases in enzymatic assays.
• Apoptosis Research: A key reagent for measuring and quantifying apoptosis in cell cultures and tissue samples.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify novel compounds that modulate caspase activity.
• Biochemical Pathway Analysis: Facilitates the study of cell death signaling pathways in cancer and neurodegenerative disease research.
• Diagnostic Development: Serves as a core component in developing in-vitro diagnostic tests for conditions involving dysregulated apoptosis.
• Toxicology Studies: Employed to assess the cytotoxic effects of chemicals or pharmaceuticals by measuring induced caspase activation.

Basic Information

Product Name	Ac-Val-Glu-His-Asp-Afc
CAS No.	1135418-22-2
Molecular Formula	C33H42N8O12
Molecular Weight	742.74 g/mol
Synonyms	Ac-VEHD-AFC; Ac-Val-Glu-His-Asp-7-Amino-4-trifluoromethylcoumarin; Caspase-3 Substrate IV; Ac-VEHD-AFC Caspase Substrate; N-Acetyl-Val-Glu-His-Asp-7-Amino-4-trifluoromethylcoumarin; Caspase-3 Fluorogenic Substrate; Ac-DEVD-AFC Analogue; Apoptosis Detection Reagent
EINECS	Contact for details

Quality Control

Every batch of Ac-Val-Glu-His-Asp-Afc is manufactured under strict quality protocols. Our products undergo rigorous quality testing, including HPLC for purity verification and mass spectrometry for identity confirmation, to ensure compliance with research-grade standards. Certificates of Analysis (COA) detailing purity, identity, and performance in functional assays are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF
Functional Activity	Conforms to reference standard in caspase-3 assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.