Description

4-Nitrophenyl o-6-Deoxy-α-L-Galactopyranosyl-(1-2)-o-β-D-Galactopyranosyl-(1-3)-2-(Acetylamino)-2-Deoxy-α-D-Galactopyranoside is a complex, synthetic oligosaccharide derivative featuring a 4-nitrophenyl aglycone. This compound is of significant value as a highly specific chromogenic substrate for the detection and kinetic study of glycosyl hydrolase enzymes, particularly α-galactosidases. It is primarily utilized by research institutions, diagnostic kit manufacturers, and pharmaceutical R&D departments engaged in enzymology, glycobiology, and the development of assays for genetic disorders like Fabry disease.

Application

• Enzymatic Research Substrate: A critical tool for studying the activity, specificity, and inhibition of α-galactosidase and related enzymes in vitro.
• Diagnostic Assay Development: Used in the formulation of colorimetric or fluorogenic kits for the clinical diagnosis of lysosomal storage diseases and enzyme deficiency disorders.
• Biochemical Reagent: Serves as a standard or reference compound in glycobiology research for investigating carbohydrate-protein interactions and metabolic pathways.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize potential therapeutic enzyme inhibitors or activators.
• Quality Control in Biologics: Can be used to assay the enzymatic activity of recombinant enzyme preparations used in therapeutic applications.

Basic Information

Product Name	4-Nitrophenyl o-6-Deoxy-α-L-Galactopyranosyl-(1-2)-o-β-D-Galactopyranosyl-(1-3)-2-(Acetylamino)-2-Deoxy-α-D-Galactopyranoside
CAS No.	1105508-81-3
Molecular Formula	C32H46N2O21
Molecular Weight	794.71 g/mol
Synonyms	4-Nitrophenyl α-D-Galactopyranosyl-(1→2)-β-D-galactopyranosyl-(1→3)-2-acetamido-2-deoxy-α-D-galactopyranoside; p-Nitrophenyl-6-deoxy-α-L-galactopyranosyl-(1→2)-β-D-galactopyranosyl-(1→3)-2-acetamido-2-deoxy-α-D-galactopyranoside; 4-Nitrophenyl α-Gal-(1→2)-β-Gal-(1→3)-α-GalNAc; PNP-α-D-Galp-(1→2)-β-D-Galp-(1→3)-α-D-GalNAc; Synthetic Trisaccharide Substrate; α-galactosidase chromogenic substrate; Fabry disease assay substrate
EINECS	Contact for details

Quality Control

Every batch of our 4-Nitrophenyl trisaccharide substrate is manufactured and analyzed under strict quality protocols to ensure high purity and batch-to-batch consistency, critical for reproducible research and diagnostic results. Our quality assurance includes comprehensive testing via HPLC, NMR, and MS. A detailed Certificate of Analysis (COA) providing purity, identification, and impurity profiles is supplied with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Specific Rotation	Contact for details
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.