Description

1,2-Distearoyl-Sn-Glycero-3-Phosphoethanolamine-n-[Poly(Ethylene Glycol)2000-N'-Carboxyfluorescein] (Ammonium Salt) is a high-purity, fluorescently labeled phospholipid-PEG conjugate, specifically designed for advanced biomedical research and diagnostic applications. This compound is critical for creating targeted delivery systems and imaging probes due to its unique combination of a lipid anchor, a biocompatible PEG spacer, and a sensitive carboxyfluorescein reporter. It is an essential reagent for pharmaceutical developers, academic researchers, and diagnostic kit manufacturers working in liposome formulation, cell membrane studies, and in vivo imaging.

Application

• Fluorescent Liposome & Nanoparticle Labeling: Essential for tracking the cellular uptake, biodistribution, and stability of lipid-based drug delivery vehicles.
• Cell Membrane & Lipid Raft Studies: Used as a fluorescent probe to visualize and study membrane dynamics, fusion, and structure in live-cell imaging.
• Targeted Drug Delivery Systems: Incorporated into the lipid bilayer of targeted liposomes to enable real-time, fluorescent monitoring of drug carrier localization.
• In Vitro & In Vivo Imaging Agents: Serves as a key component in the development of contrast agents for fluorescence microscopy and non-invasive imaging techniques.
• Bioconjugation & Surface Modification: The reactive carboxyfluorescein group allows for further conjugation to antibodies, peptides, or other targeting ligands.
• Quality Control in Liposome Manufacturing: Acts as a tracer to monitor encapsulation efficiency, particle size distribution, and batch-to-batch consistency.
• Apoptosis & Cytotoxicity Assays: Utilized in assays that detect changes in membrane phospholipid distribution during cell death pathways.

Basic Information

Product Name	1,2-Distearoyl-Sn-Glycero-3-Phosphoethanolamine-n-[Poly(Ethylene Glycol)2000-N'-Carboxyfluorescein] (Ammonium Salt)
CAS No.	1092697-61-4
Molecular Formula	C₁₃₀H₂₄₈N₂O₅₈P (Approximate, for PEG2000 average)
Molecular Weight	~2800 g/mol (Average)
Synonyms	DSPE-PEG(2000)-Carboxyfluorescein; DSPE-PEG2000-CF; 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[carboxy(polyethylene glycol)-2000] (ammonium salt) labeled with carboxyfluorescein; Fluorescent PEGylated phospholipid; CF-DSPE-PEG2000; PEG-DSPE-Carboxyfluorescein; PE-PEG(2000)-CF; Phosphoethanolamine-PEG-Carboxyfluorescein conjugate
EINECS	Contact for details

Quality Control

Every batch of our 1,2-Distearoyl-Sn-Glycero-3-Phosphoethanolamine-n-[Poly(Ethylene Glycol)2000-N'-Carboxyfluorescein] (Ammonium Salt) is manufactured under strict quality management systems and undergoes comprehensive analytical testing. We ensure compliance with research-grade and cGMP standards where applicable, utilizing advanced techniques like HPLC and mass spectrometry for verification. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming identity, purity, fluorescence properties, and functional performance.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store in aliquots under an inert atmosphere to prevent oxidation and hydrolysis. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Fluorophore Content	0.8 - 1.2 mol CF per mol phospholipid
Free Carboxyfluorescein	≤ 1.0%
Solubility	Soluble in chloroform, DMSO; forms micelles in aqueous buffer
Critical Micelle Concentration (CMC)	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.