Description

n-(2-Maleimidoethyl)-6-T-Boc-Aminohexanamide is a heterobifunctional crosslinking reagent featuring a maleimide group and a protected amine. This compound is specifically engineered for the site-directed conjugation of biomolecules, such as antibodies and peptides, under controlled conditions. It is a critical intermediate for researchers and manufacturers in the fields of bioconjugation, antibody-drug conjugate (ADC) development, and diagnostic reagent formulation.

Application

• Antibody-Drug Conjugate (ADC) Linker Synthesis: Serves as a key building block for constructing cleavable or non-cleavable linkers that connect cytotoxic payloads to monoclonal antibodies.
• Protein and Peptide Labeling: Used for the site-specific modification of cysteine residues via the maleimide group, enabling the attachment of fluorophores, biotin, or other functional tags.
• Surface Immobilization Chemistry: Facilitates the oriented coupling of proteins to biosensor chips, microarrays, and nanoparticle surfaces for assay development.
• Polymer Functionalization: Enables the introduction of reactive amine handles (after deprotection) into polymer backbones for creating advanced biomaterials.
• Diagnostic Reagent Production: Employed in the manufacturing of enzyme conjugates and labeled antibodies for immunoassays (ELISA, lateral flow tests).
• Research Tool for Chemical Biology: Provides a controlled method for studying protein-protein interactions and constructing defined molecular architectures.

Basic Information

Product Name	n-(2-Maleimidoethyl)-6-T-Boc-Aminohexanamide
CAS No.	1076198-36-1
Molecular Formula	C20H33N3O6
Molecular Weight	411.50 g/mol
Synonyms	6-[(2-{[(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl]amino}-2-oxoethyl)amino]hexyl carbamic acid tert-butyl ester; N-(2-Maleimidoethyl)-6-(tert-butoxycarbonylamino)hexanamide; Maleimide-PEG2-TFA, Boc-protected; Boc-NH-PEG2-Maleimide; tert-Butyl N-[6-({2-[(2,5-dioxopyrrol-1-yl)ethyl]amino}-2-oxoethyl)amino]hexyl]carbamate; Heterobifunctional PEG crosslinker, Boc-protected; 1-[2-({6-[(tert-Butoxycarbonyl)amino]hexanoyl}amino)ethyl]-1H-pyrrole-2,5-dione
EINECS	Contact for details

Quality Control

Our n-(2-Maleimidoethyl)-6-T-Boc-Aminohexanamide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure it meets the high standards required for sensitive bioconjugation applications. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to warm to room temperature before opening to minimize moisture condensation. For long-term storage, keep containers in a dry, cold environment.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.